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Mucilaginibacter calamicampi sp. nov., a member of the family Sphingobacteriaceae isolated from soil at a field of reeds

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Abstract

A Gram-negative, aerobic, non-flagellated, non-gliding, rod-shaped bacterial strain, designated WR-R1YT, was isolated from soil at a field of reeds in South Korea. Strain WR-R1YT grew optimally at 30 °C, at pH 7.0–7.5 and in the absence of NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain WR-R1YT fell within the clade comprising Mucilaginibacter species, coherently clustering with the type strain of Mucilaginibacter composti, with which it exhibited the highest 16S rRNA gene sequence similarity value of 97.6 %. Sequence similarities to the type strains of the other Mucilaginibacter species and the other species used in the phylogenetic analysis were 93.1–96.9 % and <91.1 %, respectively. Strain WR-R1YT contained MK-7 as the predominant menaquinone and iso-C15:0, summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), C16:0 and iso-C17:0 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified aminophospholipid. The DNA G+C content of strain WR-R1YT was 43.1 mol% and its mean DNA–DNA relatedness value with M. composti KACC 14956T was 17 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain WR-R1YT is separate from other Mucilaginibacter species. On the basis of the data presented, strain WR-R1YT represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter calamicampi sp. nov. is proposed. The type strain is WR-R1YT (= KCTC 32214T = CCUG 63418T).

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Acknowledgments

This work was supported by the Program for Collection of Domestic Biological Resources from the National Institute of Biological Resources (NIBR) and the Program for Collection, Management and Utilization of Biological Resources and BK 21 program from the Ministry of Education, Science and Technology (MEST) of the Republic of Korea. We are very grateful to the KACC for kindly providing the type strains of Mucilaginibacter species.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Jung-Hoon Yoon, Sooyeon Park & Yong-Taek Jung

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  1. Jung-Hoon Yoon
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  2. Sooyeon Park
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  3. Yong-Taek Jung
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Correspondence to Jung-Hoon Yoon.

Additional information

The GenBank/EMBL/DDBJ accession no. for the 16S rRNA gene sequence of strain WR-R1YT is KC537738.

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Yoon, JH., Park, S. & Jung, YT. Mucilaginibacter calamicampi sp. nov., a member of the family Sphingobacteriaceae isolated from soil at a field of reeds. Antonie van Leeuwenhoek 104, 37–45 (2013). https://doi.org/10.1007/s10482-013-9922-4

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