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Nocardioides panaciterrulae sp. nov., isolated from soil of a ginseng field, with ginsenoside converting activity

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Abstract

A Gram-positive, coccoid to rod-shaped, non-spore-forming bacterium, designated Gsoil 958T, was isolated from soil of a ginseng field located in Pocheon province in South Korea. This bacterium was characterized in order to determine its taxonomic position by using a polyphasic approach. Strain Gsoil 958T was observed to grow well at 25–30 °C and at pH 7.0 on R2A and nutrient agar without NaCl supplementation. Strain Gsoil 958T was determined to have β-glucosidase activity and the ability to transform ginsenoside Rb1 (one of the dominant active components of ginseng) to F2 via gypenoside XVII and Rd. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 958T was shown to belong to the family Nocardioidaceae and related most closely to Nocardioides koreensis MSL-09T (97.6 % 16S rRNA gene sequence similarity), Nocardioides aquiterrae GW-9T (97.0 %), and Nocardioides sediminis MSL-01T (97.0 %). The sequence similarities with other validly named species within the genus Nocardioides were less than 96.8 %. Strain Gsoil 958T was characterized chemotaxonomically as having LL-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone, and iso-C16:0, iso-C16:1 H, iso-C14:0, iso-C15:0 were identified as the major fatty acids. The G + C content of genomic DNA was determined to be 70.8 mol %. The chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain Gsoil 958T to the genus Nocardioides. The results of both physiological and biochemical tests allowed for differentiation of strain Gsoil 958T from the recognized Nocardioides species. Therefore, strain Gsoil 958T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides panaciterrulae sp. nov. is proposed, with the type strain Gsoil 958T (KACC 14271T = KCTC 19471T = DSM 21350T).

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Acknowledgments

This work was supported by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Education, Science and Technology (2011-0031967) and by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment, Korea.

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Authors and Affiliations

  1. KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Jin-Kwang Kim, Qing-Mei Liu, Sun-Chang Kim & Wan-Taek Im

  2. Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 305-764, Republic of Korea

    Jin-Kwang Kim & Min-Ho Yoon

  3. Intelligent Synthetic Biology Center, 373-1, Guseong-dong, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Qing-Mei Liu & Sun-Chang Kim

  4. Microorganism Resources Division, National Institute of Biological Resources, Incheon, 404-708, Republic of Korea

    Hye-Yoon Park & Myung-Suk Kang

  5. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Sun-Chang Kim

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  1. Jin-Kwang Kim
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  2. Qing-Mei Liu
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  3. Hye-Yoon Park
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Correspondence to Wan-Taek Im or Min-Ho Yoon.

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Kim, JK., Liu, QM., Park, HY. et al. Nocardioides panaciterrulae sp. nov., isolated from soil of a ginseng field, with ginsenoside converting activity. Antonie van Leeuwenhoek 103, 1385–1393 (2013). https://doi.org/10.1007/s10482-013-9919-z

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