Saccharopolyspora dendranthemae sp. nov. a halotolerant endophytic actinomycete isolated from a coastal salt marsh plant in Jiangsu, China

Abstract

A halotolerant actinomycete strain, designated strain KLBMP 1305T, was isolated from a salt marsh plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KLBMP 1305T is a member of the genus Saccharopolyspora. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1305T was most closely related to ‘Saccharopolyspora pathumthaniensis’ S582T (99.31 %), ‘Saccharopolyspora endophytica’ YIM 61095T (99.17 %) and Saccharopolyspora tripterygii YIM 65359T (99.15 %); similarity to other type strains of the genus Saccharopolyspora was <97.2 %. The organism had chemical and morphological features consistent with its classification in the genus Saccharopolyspora such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The predominant menaquinone was MK-9(H4). The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unknown glycolipid and an unknown lipid. The major fatty acids were iso-C16:0, iso-C15:0, anteiso-C15:0, anteiso-C17:0 and sum in feature 8 (18:1ω7c/18:1ω6c). The G+C content of the genomic DNA of the type strain was 68.7 mol%. DNA–DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate represents a novel species, for which the name Saccharopolyspora dendranthemae sp. nov. is proposed. The type strain is KLBMP 1305T (=KCTC 19889T = NBRC 108675T).

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Acknowledgments

The authors are grateful to Prof. Tomohiko Tamura (NITE Biological Resource Center, NBRC) for kindly providing the type strains, Prof. Jean Euzeby for the help of the nomenclature and Prof. Martha E. Trujillo for his valuable comments on the manuscript. This research was partially supported by National Natural Science Foundation of China (31000005, 31101502), the Program of Natural Science Foundation of the Jiangsu Higher Education Institutions of China (11KJD210002), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and the innovative research project of Jiangsu Normal University (2012YYB091).

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Correspondence to Sheng Qin or Ji-Hong Jiang.

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Yue-Ji Zhang and Wen-Di Zhang contributed equally to this study.

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Zhang, YJ., Zhang, WD., Qin, S. et al. Saccharopolyspora dendranthemae sp. nov. a halotolerant endophytic actinomycete isolated from a coastal salt marsh plant in Jiangsu, China. Antonie van Leeuwenhoek 103, 1369–1376 (2013). https://doi.org/10.1007/s10482-013-9917-1

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Keywords

  • Saccharopolyspora dendranthemae sp. nov.
  • Coastal halophyte
  • Endophytic
  • Polyphasic taxonomy