Litorimonas cladophorae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Cladophora stimpsoni, and emended descriptions of the genus Litorimonas and Litorimonas taeaensis

Abstract

A strictly aerobic, Gram-stain-negative, rod-shaped and red-orange pigmented bacterium, designated strain KMM 6395T, was isolated from the green alga Cladophora stimpsoni and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the novel strain affiliated to the family Hyphomonadaceae of the class Alphaproteobacteria being most closely related to the type strain of the single species of the genus Litorimonas, Litorimonas taeanensis G5T, with 16S rRNA gene sequence similarity of 96.8 %. Strain KMM 6395T grew with 1–5 % NaCl and at 4–35 °C, hydrolysed starch and Tween 80. The DNA G+C content was 48.7 mol%. The prevalent fatty acids were C18:1 ω7c, C19:1 ω8c and C18:1 ω7c 10-methyl. The polar lipid profile was characterized by the presence of phosphatidylglycerol, monoglycosyldiglyceride, glucuronopyranosyldiglyceride and an unidentified glycolipid. The major respiratory quinone was Q-10. The significant molecular distinctiveness between the novel isolate and its nearest neighbour, L. taeanensis G5T, were strongly supported by the differences in physiological and biochemical tests. Therefore, strain KMM 6395T represents a novel species of the genus Litorimonas, for which the name Litorimonas cladophorae sp. nov. is proposed. The type strain is KMM 6395T (=KCTC 23968T = LMG 26985T). The emended descriptions of the genus Litorimonas and L. taeaensis are also provided.

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Acknowledgments

We thank Dr. Ji Young Jung (Chung-Ang University, Seoul, Republic of Korea) for providing us with the type strain L. taenensis G5T. Dr. D.V. Fomin (Cooperative Far Eastern Center of Electron Microscopy, Vladivostok, Russia) is gratefully acknowledged for his excellent technical assistance. This research was supported by grants of the Presidium of the Russian Academy of Sciences “Molecular and Cell Biology”, the Presidium of the Far-Eastern Branch of the Russian Academy of Sciences no. 12-III-A-06-105, the government of Russian Federation for the state support of scientific investigations conducting under the guidance of the leading researchers at the Russian education institutions of the high professional education, agreement no. 11.G34.31.0010 and the Russian Foundation for Basic Research (RFBR) no. 11-04-00781.

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Correspondence to Olga I. Nedashkovskaya.

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Nedashkovskaya, O.I., Kukhlevskiy, A.D., Zhukova, N.V. et al. Litorimonas cladophorae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Cladophora stimpsoni, and emended descriptions of the genus Litorimonas and Litorimonas taeaensis . Antonie van Leeuwenhoek 103, 1263–1269 (2013). https://doi.org/10.1007/s10482-013-9906-4

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Keywords

  • Litorimonas cladophorae sp. nov.
  • Alphaproteobacteria
  • Marine bacteria
  • Phylogeny
  • Green alga Cladophora stimpsoni