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Litorimonas cladophorae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Cladophora stimpsoni, and emended descriptions of the genus Litorimonas and Litorimonas taeaensis

Antonie van Leeuwenhoek volume 103, pages 1263–1269 (2013)Cite this article

Abstract

A strictly aerobic, Gram-stain-negative, rod-shaped and red-orange pigmented bacterium, designated strain KMM 6395T, was isolated from the green alga Cladophora stimpsoni and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the novel strain affiliated to the family Hyphomonadaceae of the class Alphaproteobacteria being most closely related to the type strain of the single species of the genus Litorimonas, Litorimonas taeanensis G5T, with 16S rRNA gene sequence similarity of 96.8 %. Strain KMM 6395T grew with 1–5 % NaCl and at 4–35 °C, hydrolysed starch and Tween 80. The DNA G+C content was 48.7 mol%. The prevalent fatty acids were C18:1 ω7c, C19:1 ω8c and C18:1 ω7c 10-methyl. The polar lipid profile was characterized by the presence of phosphatidylglycerol, monoglycosyldiglyceride, glucuronopyranosyldiglyceride and an unidentified glycolipid. The major respiratory quinone was Q-10. The significant molecular distinctiveness between the novel isolate and its nearest neighbour, L. taeanensis G5T, were strongly supported by the differences in physiological and biochemical tests. Therefore, strain KMM 6395T represents a novel species of the genus Litorimonas, for which the name Litorimonas cladophorae sp. nov. is proposed. The type strain is KMM 6395T (=KCTC 23968T = LMG 26985T). The emended descriptions of the genus Litorimonas and L. taeaensis are also provided.

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References

  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    PubMed  Article  CAS  Google Scholar 

  • Collins MD, Shah HM (1984) Fatty acid, menaquinone and polar lipid composition of Rothia dentosacariosa. Arch Microbiol 137:247–249

    Article  CAS  Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    PubMed  Article  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Gonzalez JM, Saiz-Jimenez C (2002) A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773

    PubMed  Article  CAS  Google Scholar 

  • Jung JY, Kim JM, Jin HM, Kim SY, Park W, Jeon CO (2011) Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach. Int J Syst Evol Microbiol 61:1534–1538

    PubMed  Article  CAS  Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    PubMed  Article  CAS  Google Scholar 

  • Komagata K, Suzuki K-I (1987) Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207

    Article  CAS  Google Scholar 

  • Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175

    Google Scholar 

  • Lemos ML, Toranzo AE, Barja JL (1985) Modified medium for oxidation-fermentation test in the identification of marine bacteria. Appl Environ Microbiol 40:1541–1543

    Google Scholar 

  • Nedashkovskaya OI, Kim SB, Han SK, Rhee MS, Lysenko AM, Falsen E, Frolova GM, Mikhailov VV, Bae KS (2004) Ulvibacter litoralis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the green alga Ulva fenestrata. Int J Syst Evol Microbiol 54:119–123

    PubMed  Article  CAS  Google Scholar 

  • Pruesse E, Quast C, Knittel K, Fuchs BM, Ludwig W, Peplies J, Glockner FO (2007) SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res 35:7188–7196

    PubMed  Article  CAS  Google Scholar 

  • Rzhetsky A, Nei M (1992) A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 9:945–967

    CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Sambrook J, Russell DW (2001) Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16

    Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    PubMed  Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr. Ji Young Jung (Chung-Ang University, Seoul, Republic of Korea) for providing us with the type strain L. taenensis G5T. Dr. D.V. Fomin (Cooperative Far Eastern Center of Electron Microscopy, Vladivostok, Russia) is gratefully acknowledged for his excellent technical assistance. This research was supported by grants of the Presidium of the Russian Academy of Sciences “Molecular and Cell Biology”, the Presidium of the Far-Eastern Branch of the Russian Academy of Sciences no. 12-III-A-06-105, the government of Russian Federation for the state support of scientific investigations conducting under the guidance of the leading researchers at the Russian education institutions of the high professional education, agreement no. 11.G34.31.0010 and the Russian Foundation for Basic Research (RFBR) no. 11-04-00781.

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Authors and Affiliations

  1. G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022, Vladivostok, Russia

    Olga I. Nedashkovskaya

  2. A.V. Zhirmunsky Institute of Marine Biology, Far-Eastern Branch, Russian Academy of Sciences, Pal’chevskogo Street 17, 690032, Vladivostok, Russia

    Andrey D. Kukhlevskiy & Natalia V. Zhukova

  3. Far Eastern Federal University, Sukhanova Street 8, 690950, Vladivostok, Russia

    Andrey D. Kukhlevskiy & Natalia V. Zhukova

  4. Department of Microbiology, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    So-Jeong Kim & Sung-Keun Rhee

Authors
  1. Olga I. Nedashkovskaya
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  2. Andrey D. Kukhlevskiy
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  3. Natalia V. Zhukova
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  4. So-Jeong Kim
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  5. Sung-Keun Rhee
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Correspondence to Olga I. Nedashkovskaya.

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Nedashkovskaya, O.I., Kukhlevskiy, A.D., Zhukova, N.V. et al. Litorimonas cladophorae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Cladophora stimpsoni, and emended descriptions of the genus Litorimonas and Litorimonas taeaensis . Antonie van Leeuwenhoek 103, 1263–1269 (2013). https://doi.org/10.1007/s10482-013-9906-4

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Keywords

  • Litorimonas cladophorae sp. nov.
  • Alphaproteobacteria
  • Marine bacteria
  • Phylogeny
  • Green alga Cladophora stimpsoni