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Glaciecola aquimarina sp. nov., a gammaproteobacterium isolated from coastal seawater

Abstract

A Gram-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, GGW-M5T, was isolated from seawater on the southern coast in Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain GGW-M5T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain GGW-M5T belonged to the genus Glaciecola, joining the cluster comprising the type strains of G. agarilytica, G. arctica, G. chathamensis, G. mesophila, G. polaris and G. psychrophila, with which it exhibited 16S rRNA gene sequence similarity values of 95.9–96.7 %. Strain GGW-M5T exhibited sequence similarity values of 93.2–94.8 % to the type strains of the other Glaciecola species. Strain GGW-M5T contained Q-8 as the predominant ubiquinone and C16:1 ω7c and/or iso-C15:0 2-OH, C16:0 and C14:0 2-OH as the major fatty acids. Major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. The DNA G+C content was 42.4 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain GGW-M5T could be distinguished from other Glaciecola species. On the basis of the data presented, strain GGW-M5T is considered to represent a novel species of the genus Glaciecola, for which the name Glaciecola aquimarina sp. nov. is proposed. The type strain is GGW-M5T (=KCTC 32108T = CCUG 62918T).

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Acknowledgments

This work was supported by the Program for Collection, Management and Utilization of Biological Resources and the BK21 program from the Ministry of Education, Science and Technology (MEST) of the Republic of Korea.

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Correspondence to Jung-Hoon Yoon.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain GGW-M5T is JX508596. Transmission electron micrograph of cell of strain GGW-M5T and thin layer chromatograms of the polar lipids of strain GGW-M5T and Glaciecola punicea LMG 21426T are available as supplementary figures in the online version of this paper.

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Park, S., Yoon, JH. Glaciecola aquimarina sp. nov., a gammaproteobacterium isolated from coastal seawater. Antonie van Leeuwenhoek 103, 1141–1148 (2013). https://doi.org/10.1007/s10482-013-9896-2

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Keywords

  • Glaciecola aquimarina sp. nov.
  • Novel species
  • Polyphasic taxonomy
  • Seawater