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Streptomyces tunisiensis sp. nov., a novel Streptomyces species with antibacterial activity

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Abstract

A novel actinomycete strain designated CN-207T was isolated from northern Tunisian soil. This strain exhibited potent broad spectrum antibacterial activity against clinical isolates of methicillin-resistant Staphylococcus species and several other Gram-positive and Gram-negative bacteria. Strain CN-207T developed greyish aerial mycelium and pale grey substrate mycelium on yeast extract/malt agar. The isolate produced branching vegetative mycelia with sporangiophores bearing sporangia developing at a late stage of growth. The sporangia contained smooth, non-motile spores. Chemotaxonomic characteristics of strain CN-207T were typical of the Streptomyces genus. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CN-207T belonged to the genus Streptomyces, and was most closely related to Streptomyces griseoincarnatus DSM 40274T, Streptomyces variabilis DSM 40179T, Streptomyces labedae DSM 41446T and Streptomyces erythrogriseus DSM 40116T. Low DNA–DNA relatedness values were recorded between strain CN-207T and its closest phylogenetic neighbours. Strain CN-207T was also distinguished from the nearest phylogenetic neighbours using a combination of morphological and phenotypic characteristics. On the basis of its phenotypic and molecular properties, strain CN-207T is considered as a novel species of the Streptomyces genus, for which the name Streptomyces tunisiensis sp. nov. is proposed. The type strain is CN-207T (=JCM 17589T = DSM 42037T).

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Acknowledgments

We are grateful to the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Braunschweig, Germany) for the DNA-DNA hybridization and Macrogen DNA sequencing service (Inc. Netherland) for the 16S rRNA gene sequence sequencing. We are also grateful to Mr Majdi Hammami for his technical assistance in fatty acid analyses (Laboratoire des Substances Bioactives). This work was supported by the Ministère de l’Enseignement Supérieur et de la Recherche Scientifique of Tunisia. We would like to thank Prof Assia Belhassen for kindly providing clinical isolates of Staphylococcus species and Prof. Ezzedine Aouani for critically reading the manuscript.

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Authors and Affiliations

  1. Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj Cedria, 901, 2050, Hammam-lif, Tunisia

    Nedra Slama, Houda Mankai, Ameni Ayed & Ferid Limam

  2. UR04CNSTN01, Applications Médicales et Agricoles des Techniques Nucléaires, Centre National des Sciences et des Technologies Nucléaires, Technopole de Sidi Thabet, 2020, Tunis, Tunisia

    Nedra Slama, Houda Mankai, Karim Mezhoud, Hadeer Lazim & Insaf Barkallah

  3. Service commun de microscopie électronique des laboratoires des Sciences de la vie du MNHN, 12 Rue Buffon, 75005, Paris, France

    Catherine Rauch

  4. Laboratoire des Microorganismes et Biomolécules Actives, Département de Biologie, Faculté des Sciences de Tunis, Université Tunis El-Manar, Tunis, Tunisia

    Maher Gtari

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  1. Nedra Slama
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Slama, N., Mankai, H., Ayed, A. et al. Streptomyces tunisiensis sp. nov., a novel Streptomyces species with antibacterial activity. Antonie van Leeuwenhoek 105, 377–387 (2014). https://doi.org/10.1007/s10482-013-0086-z

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