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Deinococcus radiotolerans sp. nov., a gamma-radiation-resistant bacterium isolated from gamma ray-irradiated soil

Abstract

Two gamma-radiation-resistant bacterial strains, designated C1T and C2, were isolated from a soil sample collected at Jeongeup-Si, South Korea. These strains were observed to be Gram-negative, non-motile, rod-shaped, and to form pink colonies. Phylogenetic analysis based on 16S rRNA gene sequences revealed that these strains belong to the genus Deinococcus in the family Deinococcaceae. Strains C1T and C2 have the highest sequence similarities with Deinococcus daejeonensis MJ27T (97.56 %) and Deinococcus grandis DSM 39663T (97.50 %). Like other members of the genus Deinococcus, the novel isolates showed resistance to gamma-radiation with a D10 value in excess of 8 kGy. The isolates were found to have menaquinone MK-8 as the predominant respiratory quinone and an unidentified phosphoglycolipid as major polar lipid. In addition, the most abundant fatty acids of strain C1T were identified as C15:1 ω6c (25.5 %), C16:1 ω7c (18.7 %) and C15:0 (9.7 %). Genomic analysis results showed that the DNA G+C contents of strain C1T and C2 are 68.59 and 68.57 %, respectively. Taken together, the polyphasic taxonomic data support the proposal that the isolates C1T and C2 represent a novel species of the genus Deinococcus, for which the name Deinococcus radiotolerans sp. nov. is proposed. The type strain is a strain C1T (=KCTC 33150T = JCM 19173T).

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Acknowledgments

This work was supported by a special research grant from Seoul Women’s University (2014). We are grateful to Dr. Jean Euzéby for help with etymology, and to Dr. Sang-Yong Lim and Mr. Min-Ho Joe in the Korea Atomic Energy Research Institute for their help with gamma-radiation resistance testing.

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Correspondence to Taegun Seo or Myung Kyum Kim.

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Cha, S., Srinivasan, S., Seo, T. et al. Deinococcus radiotolerans sp. nov., a gamma-radiation-resistant bacterium isolated from gamma ray-irradiated soil. Antonie van Leeuwenhoek 105, 229–235 (2014). https://doi.org/10.1007/s10482-013-0069-0

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Keywords

  • Deinococcaceae
  • Deinococci
  • Deinococcus
  • Radiation
  • Taxonomy