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Nonomuraea shaanxiensis sp. nov., a novel actinomycete isolated from a soil sample

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Abstract

A novel actinomycete, strain NEAU-st1T, was isolated from a soil sample collected in Shaanxi province, Northwest China and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-st1T belongs to the genus Nonomuraea, being most closely related to Nonomuraea rosea GW12687T (98.91 %), Nonomuraea solani NEAU-Z6T (98.44 %), Nonomuraea rhizophila YIM67092T(98.24 %) and Nonomuraea monospora PT708T (98.02 %); similarities to sequences of other type strains of the genus Nonomuraea were lower than 98 %. Both tree-making algorithms used also supported the position that strain NEAU-st1T formed a distinct clade with its most closely related species. Morphological and physiological characteristics confirmed that the strain belongs to the genus Nonomuraea and distinguished it from its most closely related species. DNA–DNA hybridization further differentiated strain NEAU-st1T from its nearest phylogenetic neighbours. These results suggested that strain NEAU-st1T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea shaanxiensis sp. nov. is proposed. The type strain is NEAU-st1T (=CGMCC 4.7096T = DSM 45877T).

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Acknowledgments

This work was supported in part by grants from the National Key Project for Basic Research (No. 2010CB126102) and the National Natural Science Foundation of China (No. 30971937 and 30771427), the National Outstanding Youth Foundation (No. 31225024), the Special Foundation for Scientific and Technological Innovation Research of Harbin (No. 2011RFXXN038), the Natural Science Foundation of Heilongjiang Province (No. C201029).

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Authors and Affiliations

  1. School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Yuejing Zhang, Junwei Zhao, Chongxi Liu, Feiyu Jia & Wensheng Xiang

  2. Key Laboratory of Microbial Drug Engineering of Heilongjiang Provincial Education Committee, Harbin, 150030, People’s Republic of China

    Yue Shen & Xiangjing Wang

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  1. Yuejing Zhang
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  2. Junwei Zhao
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  3. Chongxi Liu
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  7. Wensheng Xiang
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Correspondence to Xiangjing Wang or Wensheng Xiang.

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Zhang, Y., Zhao, J., Liu, C. et al. Nonomuraea shaanxiensis sp. nov., a novel actinomycete isolated from a soil sample. Antonie van Leeuwenhoek 105, 57–64 (2014). https://doi.org/10.1007/s10482-013-0052-9

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