Wohlfahrtiimonas larvae sp. nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae)

Antonie van Leeuwenhoek volume 105, pages15–21(2014)Cite this article

Abstract

A novel, Gram-negative, facultative anaerobic, motile and short rod-shaped bacterium, strain KBL006T was isolated from the larval gut of Hermetia illucens, Black soldier fly. The 16S rRNA gene sequence of strain KBL006T showed 96.4 % similarity to that of Wohlfahrtiimonas chitiniclastica S5T. Strain KBL006T grew optimally at 30 °C, at pH 8.0 and in the presence of 1–2 % (w/v) NaCl. Oxidase activity and catalase activity were positive. The major fatty acids were C18:1 ω7c, C14:0, and C16:0. The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol, and two phospholipids. The G+C content of the genomic DNA was 45.2 mol%. Based on these polyphasic data, strain KBL006T is considered to represent a novel species in the genus Wohlfahrtiimonas, for which the name Wohlfahrtiimonas larvae sp. nov. is proposed. The type strain is KBL006T (= KACC 16839T = JCM 18424T).

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Fig. 1
Fig. 2

Abbreviations

DPG:

Diphosphatidylglycerol

PE:

Phosphatidylethanolamine

PG:

Phosphatidylglycerol

PL1 and PL2:

Unknown phospholipids

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Acknowledgments

We thank Drs. Jin-Woo Bae and Seong Woon Roh for experimental advices. The G + C content analysis was done at Jeju center, Korean Basic Sciences Institute. This work was supported by the Rural Development Administration in Korea (PJ006893). JK Lee thanks to graduate student scholarship of Lotte foundation. SJ Lee thanks to support by NRF 2010-0010779.

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Affiliations

  1. Department of Biology, Kyung Hee University, Seoul, 130-701, South Korea

    Jae Kook Lee, Youn Yeop Lee & Sung-Jae Lee

  2. Insect Resources Division, Department of Agricultural Biology, National Academy of Agricultural Science and Technology, Rural Development Administration, Suwon, 441-853, South Korea

    Kwan Ho Park & Youngcheol Choi

  3. Department of Visual Optics, Baekseok University, Cheonan, 330-704, South Korea

    Jeonggu Sim

Authors
  1. Jae Kook Lee
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  2. Youn Yeop Lee
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  3. Kwan Ho Park
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  4. Jeonggu Sim
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  5. Youngcheol Choi
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  6. Sung-Jae Lee
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Corresponding author

Correspondence to Sung-Jae Lee.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KBL006T is JN873914.

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Lee, J.K., Lee, Y.Y., Park, K.H. et al. Wohlfahrtiimonas larvae sp. nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae). Antonie van Leeuwenhoek 105, 15–21 (2014). https://doi.org/10.1007/s10482-013-0048-5

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Keywords

  • Wohlfahrtiimonas larvae
  • Hermetia illucens
  • Larval gut microorganism
  • Polyphasic taxonomy