A novel, Gram-negative, facultative anaerobic, motile and short rod-shaped bacterium, strain KBL006T was isolated from the larval gut of Hermetia illucens, Black soldier fly. The 16S rRNA gene sequence of strain KBL006T showed 96.4 % similarity to that of Wohlfahrtiimonas chitiniclastica S5T. Strain KBL006T grew optimally at 30 °C, at pH 8.0 and in the presence of 1–2 % (w/v) NaCl. Oxidase activity and catalase activity were positive. The major fatty acids were C18:1 ω7c, C14:0, and C16:0. The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol, and two phospholipids. The G+C content of the genomic DNA was 45.2 mol%. Based on these polyphasic data, strain KBL006T is considered to represent a novel species in the genus Wohlfahrtiimonas, for which the name Wohlfahrtiimonas larvae sp. nov. is proposed. The type strain is KBL006T (= KACC 16839T = JCM 18424T).
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We thank Drs. Jin-Woo Bae and Seong Woon Roh for experimental advices. The G + C content analysis was done at Jeju center, Korean Basic Sciences Institute. This work was supported by the Rural Development Administration in Korea (PJ006893). JK Lee thanks to graduate student scholarship of Lotte foundation. SJ Lee thanks to support by NRF 2010-0010779.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KBL006T is JN873914.
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Lee, J.K., Lee, Y.Y., Park, K.H. et al. Wohlfahrtiimonas larvae sp. nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae). Antonie van Leeuwenhoek 105, 15–21 (2014). https://doi.org/10.1007/s10482-013-0048-5
- Wohlfahrtiimonas larvae
- Hermetia illucens
- Larval gut microorganism
- Polyphasic taxonomy