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Caldimonas meghalayensis sp. nov., a novel thermophilic betaproteobacterium isolated from a hot spring of Meghalaya in northeast India

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Abstract

While studying the microbial diversity of hot springs of North-east India we isolated a strain AK31T from the Jakrem hot spring of Meghalaya. The strain formed light yellow colonies on nutrient agar and was Gram negative, non spore-forming rods, motile with single polar flagellum. The strain was positive for oxidase and catalase and hydrolysed starch and weakly urea. The predominant cellular fatty acids were C16:0 (34.8 %), C17:0 cyclo (27.1 %), C16:1 ω7c and/or iso-C15:0 2OH (summed feature 3) (9.6 %), C10:0 3OH (8.0 %), C12:0 (5.8 %), C14:0 (5.3 %) and C18:1 ω7c (5.3 %). Strain AK31T contained ubiquinone-8 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids and one unidentified glycolipid as the polar lipids. The G + C content of the DNA of the strain AK31T was 66.7 mol%. The 16S rRNA gene sequence analysis indicated that strain AK31T was member of the genus Caldimonas and closely related to Caldimonas manganoxidans JCM 10698T and Caldimonas taiwanensis On1T with 96.9 % similarity and with Aquincola tertiaricarbonis L10T and Azohydromonas australica IAM 12664T with 96.5 and 96.4 % similarity respectively. Phylogenetic analyses indicated that the strain AK31T clustered with C. manganoxidans JCM 10698T and C. taiwanensis On1T with a phylogenetic distance of 3.25 %. Based on data from the current polyphasic study, strain AK31T is proposed as a novel species of the genus Caldimonas, for which the name Caldimonas meghalayensis sp. nov. is proposed. The type strain of C. meghalayensis is AK31T (= MTCC 11703T = JCM 18786T).

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Acknowledgments

We thank Council of Scientific and Industrial Research (CSIR), Directors of IMTECH, Chandigarh and NIO, Goa and Department of Biotechnology, Government of India for encouragement and financial assistance. We would like to thank Mr. Deepak for his help in sequencing DNA. We thank Dr. J. Euzeby for his expert suggestion for the correct species epithet and Latin etymology, and Dr. S.R. Joshi for his help in sample collection. TNRS is thankful to project OLP1201 for providing facilities and funding respectively. RK is supported by DBT-Research Associateship Program of Department of Biotechnology. IMTECH communication number is 042/2013 and NIO contribution number is 5470.

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Authors and Affiliations

  1. Microbial Type Culture Collection and Gene Bank, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, 160 036, India

    K. Rakshak, K. Ravinder,  Nupur & P. Anil Kumar

  2. CSIR-National Institute of Oceanography, Regional Centre, 176, Lawsons Bay Colony, Visakhapatnam, 530 017, India

    T. N. R. Srinivas

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  1. K. Rakshak
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  2. K. Ravinder
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  3. Nupur
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  4. T. N. R. Srinivas
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  5. P. Anil Kumar
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Corresponding author

Correspondence to P. Anil Kumar.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain AK31T is HF562216.

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Rakshak, K., Ravinder, K., Nupur et al. Caldimonas meghalayensis sp. nov., a novel thermophilic betaproteobacterium isolated from a hot spring of Meghalaya in northeast India. Antonie van Leeuwenhoek 104, 1217–1225 (2013). https://doi.org/10.1007/s10482-013-0043-x

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