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Desulfotomaculum tongense sp. nov., a moderately thermophilic sulfate-reducing bacterium isolated from a hydrothermal vent sediment collected from the Tofua Arc in the Tonga Trench

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Abstract

A novel, strictly anaerobic, moderately thermophilic, endospore-forming, sulfate-reducing bacterium, designated TGB60-1T, was isolated from a hydrothermal sediment vent collected from the Tofua Arc in the Tonga Trench. The strain was characterized phenotypically and phylogenetically. The isolated strain was observed to be Gram-positive, with slightly curved rod-shaped cells and a polar flagellum. Strain TGB60-1T was found to grow anaerobically at 37–60 °C (optimum, 50 °C), at pH 6.0–8.5 (optimum, pH 7.0) and with 1.0–4.0 % (w/v) NaCl (optimum, 3.0 %). The electron acceptors utilised were determined to be sulfate, sulfite, and thiosulfate. Strain TGB60-1T was found to utilise pyruvate and H2 as electron donors. Strain TGB60-1T was determined to be related to representatives of the genus Desulfotomaculum and the closest relatives within this genus were identified as Desulfotomaculum halophilum SEBR 3139T, Desulfotomaculum alkaliphilum S1T and Desulfotomaculum peckii LINDBHT1T (92.7, 92.1, and 91.8 % 16S rRNA gene sequence similarity, respectively). The major fatty acids (>20 %) were identified as C16:0 and C18:1 ω7c. The G+C content of the genomic DNA of this novel bacterium was determined to be 53.9 mol%. Based on this polyphasic taxonomic study, strain TGB60-1T is considered to represent a novel species in the genus Desulfotomaculum, for which the name Desulfotomaculum tongense sp. nov. is proposed. The type strain of D. tongense is strain TGB60-1T (= KTCT 4534T = JCM 18733T).

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Acknowledgments

This work was supported by a grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) (NIBR No. 2013-02-055), the R & D project ‘Exploration of Seafloor Hydrothermal Deposits in Tongan Waters (PM57063)’ funded by the Ministry of Oceans and Fisheries of Korea, the Marine and Extreme Genome Research Center Program of the Ministry of Land, Transportation, and Maritime Affairs, Republic of Korea, and BK21 Plus program.

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Authors and Affiliations

  1. Department of Microbiology, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    In-Tae Cha, So-Jeong Kim, Hee-Ji Hong & Sung-Keun Rhee

  2. Jeju Center, Korea Basic Science Institute, Jeju, 690-756, Republic of Korea

    In-Tae Cha, Seong Woon Roh & Hae-Won Lee

  3. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Republic of Korea

    Wan-Taek Lim

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  1. In-Tae Cha
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Correspondence to Sung-Keun Rhee.

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In-Tae Cha and Seong Woon Roh contributed equally to this work.

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Cha, IT., Roh, S.W., Kim, SJ. et al. Desulfotomaculum tongense sp. nov., a moderately thermophilic sulfate-reducing bacterium isolated from a hydrothermal vent sediment collected from the Tofua Arc in the Tonga Trench. Antonie van Leeuwenhoek 104, 1185–1192 (2013). https://doi.org/10.1007/s10482-013-0040-0

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