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Streptomyces muensis sp. nov.

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Abstract

A strain of Streptomyces, MBRL 179T, isolated from a sample from a Limestone quarry located at Hundung, Manipur, India, was characterized by polyphasic taxonomy. The strain formed a monophyletic clade with Streptomyces spinoverrucosus NBRC 14228T (16S rRNA gene sequence similarity of 99.3 %) in the Neighbour-joining tree. DNA–DNA hybridization experiment gave a DNA–DNA relatedness value of 34.7 % between MBRL 179T and S. spinoverrucosus NBRC 14228T. Strain MBRL 179T contained LL-diaminopimelic acid, xylose, glucose, and mannose in the whole cell-wall hydrolysates along with small amount of ribose. The major polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside, with other unknown phospholipids and aminophospholipid. MK-9(H6), MK-9(H8) and MK-9(H4) were the predominant menaquinones detected. The major fatty acids were anteiso-C16:0 (28.1 %), iso-C16:0 (20.3 %), C16:0 (9.4 %) and anteiso-C17:0 (8.3 %). The G+C content of the genomic DNA was 71.1 %. Based on the polyphasic experiment results, the strain MBRL 179T merits recognition as a representative of a novel species of the genus Streptomyces for which the name Streptomyces muensis sp. nov. is proposed; the type strain is MBRL 179T (=JCM 17576T = KCTC 29124T).

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Acknowledgments

The authors are grateful to Prof. Tomohiko Tamura (NBRC) for providing the reference type strains. S Nimaichand wishes to thank the University Grants Commission (UGC), Government of India, for offering him the Rajiv Gandhi National Fellowship. K Tamreihao wishes to thank the Council of Scientific and Industrial Research (CSIR), Government of India for offering him the CSIR-SRF. Y-G Zhang was supported by the West Light Foundation of the Chinese Academy of Sciences. W-J Li was also supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences. The authors also wish to thank SAIF, NEHU, Shillong for providing the SEM facility.

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Authors and Affiliations

  1. Microbial Biotechnology Research Laboratory, Department of Biochemistry, Manipur University, Canchipur, Imphal, 795003, Manipur, India

    Debananda S. Ningthoujam, Salam Nimaichand & K. Tamreihao

  2. State Biotech Hub, Department of Biochemistry, Manipur University, Canchipur, Imphal, 795003, Manipur, India

    Debananda S. Ningthoujam & Dollyca Ningombam

  3. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Salam Nimaichand, Juan Cheng, Min-Jiao Liu & Wen-Jun Li

  4. Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, People’s Republic of China

    Li Li, Yong-Guang Zhang & Wen-Jun Li

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  1. Debananda S. Ningthoujam
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Correspondence to Wen-Jun Li.

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10482_2013_35_MOESM1_ESM.docx

Two-dimensional thin-layer chromatogram of polar lipids of strain MBRL 179T Note: 1 PIM, 2 PI, 3 PG, 4 PE, 5 DPG, 6–8 PLs, 9 APL. The chromatographic conditions were as follows: Silica Gel 60 thin-layer plates (10 × 10 cm2) were spotted with 10.0 μl of a whole-cell lipid extract. Chloroform:methanol:water (65:24:4, v/v/v) was used in the first direction, and chloroform:acetic acid:methanol:water (80:18:12:5, v/v/v/v) was used in the second direction. The spray reagent was molybdophosphoric acid. (DOCX 768 kb)

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Ningthoujam, D.S., Nimaichand, S., Ningombam, D. et al. Streptomyces muensis sp. nov.. Antonie van Leeuwenhoek 104, 1135–1141 (2013). https://doi.org/10.1007/s10482-013-0035-x

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