A Gram-stain positive, facultative anaerobic, motile, spore-forming rod-shaped bacterium with peritrichous flagella, designated DX-5T, was isolated from an electroactive biofilm. Growth was observed to occur at 35–60 °C, at pH 7.0–10.0 and with 0.5–10 % (w/v) NaCl (optimum growth: 50 °C, pH 8.0 and 0.5–3 % NaCl). Cells were determined to be catalase- and oxidase-positive. The predominant respiratory quinone was identified as MK-7; the major polar lipids were determined to be diphosphatidylglycerol, phosphatidylglycerol, glycolipid, aminoglycolipid and an unidentified phospholipid; the DNA G+C content was determined to be 46.6 mol%; and the major fatty acids (>5 %) were identified as anteiso-C15:0 (33.6 %), iso-C15:0 (24.1 %) and anteiso-C17:0 (13.4 %). The phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain DX-5T should be assigned to the genus Bacillus, and was related most closely to the type strains of B. fortis DSM 16012T (96.3 %), B. composti KACC 16872T (96.3 %) and B. fordii DSM 16014T (95.8 %). Results of phenotypic, chemotaxonomic and genotypic analysis indicated that strain DX-5T represents a novel species, for which the name B. sediminis sp. nov. is proposed. The type strain is DX-5T (=CGMCC 1.12412T = KCTC 33102T).
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This work was supported by the Team Project of Guangdong Natural Science Foundation (S2011030002882 and S2012030006114), the Science and Technology Planning Project of Guangdong Province, China (2012B030800008 and 2012B010500035) and the Guangzhou Science and Technology Development Foundation, China (2011J2200039).
Zhen Yu and Yueqiang Wang contributed equally to this study.
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Yu, Z., Wang, Y., Qin, D. et al. Bacillus sediminis sp. nov., isolated from an electroactive biofilm. Antonie van Leeuwenhoek 104, 1109–1116 (2013). https://doi.org/10.1007/s10482-013-0032-0
- Bacillus sediminis sp. nov.
- Electroactive biofilm
- Polyphasic taxonomy