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Isolation and diversity of planctomycetes from the sponge Niphates sp., seawater, and sediment of Moreton Bay, Australia

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Abstract

Planctomycetes are ubiquitous in marine environment and were reported to occur in association with multicellular eukaryotic organisms such as marine macroalgae and invertebrates. Here, we investigate planctomycetes associated with the marine sponge Niphates sp. from the sub-tropical Australian coast by assessing their diversity using culture-dependent and -independent approaches based on the 16S rRNA gene. The culture-dependent approach resulted in the isolation of a large collection of diverse planctomycetes including some novel lineages of Planctomycetes from the sponge as well as sediment and seawater of Moreton Bay where this sponge occurs. The characterization of these novel planctomycetes revealed that cells of one unique strain do not possess condensed nucleoids, a phenotype distinct from other planctomycetes. In addition, a culture-independent clone library approach identified unique planctomycete 16S rRNA gene sequences closely related to other sponge-derived sequences. The analysis of tissue of the sponge Niphates sp. showed that the mesohyl of the sponge is almost devoid of microbial cells, indicating this species is in the group of ‘low microbial abundant’ (LMA) sponges. The unique planctomycete 16S rRNA gene sequences identified in this study were phylogenetically closely related to sequences from LMA sponges in other published studies. This study has revealed new insights into the diversity of planctomycetes in the marine environment and the association of planctomycetes with marine sponges.

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Acknowledgments

Research on planctomycetes in the laboratory of J.A.F. was funded by an Australian Research Council Discovery Project. We thank Dr Merrick Ekins, Dr Patricia Sutcliffe, and Dr John Hooper at Queensland Museum for the identification of the sponge Niphates sp.; Dr Kathy Townsend at Moreton Bay Research Station for her assistance in the collection of the sponge specimens; and Marinova Pty Ltd (Tasmania, Australia) for donation of a fucoidan sample. H.I was supported by the University of Queensland Research Scholarship (UQRS) and University of Queensland International Research Tuition Award (UQIRTA).

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  1. Hiroshi Izumi

    Present address: Microbiology and Biochemistry of Secondary Metabolites Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan

Authors and Affiliations

  1. School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, 4072, Australia

    Hiroshi Izumi, Evgeny Sagulenko & John A. Fuerst

  2. Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, QLD, 4072, Australia

    Richard I. Webb

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  1. Hiroshi Izumi
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Izumi, H., Sagulenko, E., Webb, R.I. et al. Isolation and diversity of planctomycetes from the sponge Niphates sp., seawater, and sediment of Moreton Bay, Australia. Antonie van Leeuwenhoek 104, 533–546 (2013). https://doi.org/10.1007/s10482-013-0003-5

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