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Salinicoccus halitifaciens sp. nov., a novel bacterium participating in halite formation

Abstract

Strain JC90T was isolated from a soda lake in Lonar, India. Strain JC90T maintains its external pH to 8.5 and participates in halite formation. Based on 16S rRNA gene sequence similarity studies, strain JC90T was found to belong to the genus Salinicoccus and is most closely related to “Salinicoccus kekensis” K164T (99.3 %), Salinicoccus alkaliphilus T8T (98.4 %) and other members of the genus Salinicoccus (<96.5 %). However Strain JC90T is <36 % related (based on DNA–DNA hybridization) with the type strains of “S. kekensis” K164T and S. alkaliphilus T8T. The DNA G+C content of strain JC90T was determined to be 46 mol %. The cell-wall amino acids were identified as lysine and glycine. Polar lipids were found to include diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl ethanolamine, an unidentified glycolipid and unidentified lipids (L1,2). Major hopanoids of strain JC90T were determined to be bacterial hopane derivatives (BHD1,2), diplopterol, diploptene and two unidentified hopanoids (UH1,2). The predominant isoprenoid quinone was identified as menaquinone (MK-6). Anteiso-C15:0 was determined to be the predominant fatty acid and significant proportions of iso-C14:0, C14:0, iso-C15:0, C16:0, iso-C16:0, iso-C17:0, anteiso-C17:0 and C18:02OH were also detected. The results of physiological and biochemical tests support the molecular evidence and allowed a clear phenotypic differentiation of strain JC90T from all other members of the genus Salinicoccus. Strain JC90T is therefore considered to represent a novel species, for which the name Salinicoccus halitifaciens sp. nov. is proposed. The type strain is JC90T (=KCTC 13894T =DSM 25286T).

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Acknowledgments

Financial assistance received from MoES, Government of India is acknowledged. UGC-BSR-RFSMS is greatly acknowledged for the Junior Research Fellowship awarded to SY. We thank Prof. J. Euzéby for his expert suggestion concerning the correct species epithet and Latin etymology.

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Correspondence to Chintalapati Sasikala.

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Ramana, C.V., Srinivas, A., Subhash, Y. et al. Salinicoccus halitifaciens sp. nov., a novel bacterium participating in halite formation. Antonie van Leeuwenhoek 103, 885–898 (2013). https://doi.org/10.1007/s10482-012-9870-4

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  • DOI: https://doi.org/10.1007/s10482-012-9870-4

Keywords

  • Salinicoccus
  • sp. nov.
  • Haloalkaliphile
  • Halite formation
  • Polyphasic taxonomy