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Isolation and characterization of a novel violacein-like pigment producing psychrotrophic bacterial species Janthinobacterium svalbardensis sp. nov

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Abstract

A bacterial strain designated JA-1, related to Janthinobacterium lividum, was isolated from glacier ice samples from the island Spitsbergen in the Arctic. The strain was tested for phenotypic traits and the most prominent appeared to be the dark red brown to black pigmentation different from the violet pigment of Janthinobacterium, Chromobacterium and Iodobacter. Phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA hybridization tests showed that strain JA-1 belongs to the genus Janthinobacterium but represents a novel lineage distinct from the two known species of this genus, J. lividum and Janthinobacterium agaricidamnosum. The DNA G + C content of strain JA-1 was determined to be 62.3 mol %. The isolate is a psychrotrophic Gram negative bacterium, rod-shaped with rounded ends, containing intracellular inclusions and one polar flagellum. On the basis of the presented results strain JA-1 is proposed as the type strain of a novel species of the genus Janthinobacterium, for which the name Janthinobacterium svalbardensis sp. nov. is proposed (JA-1T = DSM 25734, ZIM B637).

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Acknowledgments

We would like to express our gratitude to Prof. Dr. Nina Gunde Cimerman for the arctic glacier ice samples.

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Authors and Affiliations

  1. Department of Biology, Chair for Molecular Genetics and Biology of Microorganisms, University of Ljubljana, Biotechnical Faculty, Večna Pot 111, 1000, Ljubljana, Slovenia

    Jerneja Ambrožič Avguštin & Darja Žgur Bertok

  2. Department of Biology, Chair of Zoology, University of Ljubljana, Biotechnical Faculty, Večna Pot 111, 1000, Ljubljana, Slovenia

    Rok Kostanjšek

  3. Animal Science Department, Chair for Microbiology and Microbial Biotechnology, University of Ljubljana, Biotechnical Faculty, Groblje 3, 1230, Domžale, Slovenia

    Gorazd Avguštin

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  1. Jerneja Ambrožič Avguštin
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  2. Darja Žgur Bertok
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  3. Rok Kostanjšek
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  4. Gorazd Avguštin
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Correspondence to Gorazd Avguštin.

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Ambrožič Avguštin, J., Žgur Bertok, D., Kostanjšek, R. et al. Isolation and characterization of a novel violacein-like pigment producing psychrotrophic bacterial species Janthinobacterium svalbardensis sp. nov. Antonie van Leeuwenhoek 103, 763–769 (2013). https://doi.org/10.1007/s10482-012-9858-0

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