Thermus tengchongensis sp. nov., isolated from a geothermally heated soil sample in Tengchong, Yunnan, south-west China

Abstract

A Gram-stain negative aerobic bacterium, designated YIM 77924T, was isolated from a geothermally heated soil sample collected at Rehai National Park, Tengchong, Yunnan province, south-west China. Growth was found to occur from 55 to 75 °C (optimum 65 °C), pH 6.0–8.0 (optimum pH 7.0) and 0–1 % NaCl (w/v). Cells were observed to be rod-shaped and the colonies convex, circular, smooth, yellow and non-transparent. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM 77924T belongs to the genus Thermus. The 16S rRNA gene sequence similarity values between strain YIM 77924T and other species of the genus Thermus were all below 97 %. The polar lipids of strain YIM 77924T were determined to be aminophospholipid, phospholipid and glycolipid. The predominant respiratory quinone was determined to be MK-8 and the G+C content was 66.64 mol%. The major fatty acids identified were iso-C16:0, iso-C15:0, iso-C17:0 and C16:0. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain YIM 77924T is proposed to represent a novel species, Thermus tengchongensis sp. nov., in the genus Thermus. The type strain is YIM 77924T (=KCTC 32025T = CCTCC AB2012063T).

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References

  1. Bjornsdottir SH, Petursdottir SK, Hreggvidsson GO, Skirnisdottir S, Hjorleifsdottir S, Arnfinnsson J, Kristjansson JK (2009) Thermus islandicus sp. nov., a mixotrophic sulfur-oxidizing bacterium isolated from the Torfajokull geothermal area. Int J Syst Evol Microbiol 59:2962–2966

    PubMed  Article  CAS  Google Scholar 

  2. Brock TD, Freeze H (1969) Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol 98:289–297

    PubMed  CAS  Google Scholar 

  3. Cerny G (1978) Studies on aminopeptidase for the distinction of gram-negative from gram-positive bacteria. Appl Microbiol Biotechnol 5:113–122

    Article  CAS  Google Scholar 

  4. Chung AP, Rainey FA, Valente M, Nobre MF, da Costa MS (2000) Thermus igniterrae sp. nov. and Thermus antranikianii sp. nov., two new species from Iceland. Int J Syst Evol Microbiol 50:209–217

    PubMed  Article  CAS  Google Scholar 

  5. Collins MD, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470

    Article  CAS  Google Scholar 

  6. Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    PubMed  CAS  Google Scholar 

  7. da Costa MS, Rainey FA (2001) Thermaceae fam. Nov. In: Boone DR, Castenholz RW (eds) Bergey’s manual of systematic bacteriology, 2nd edn, vol 1. Springer, New York, pp 404–414

  8. da Costa MS, Rainey FA, Nobre MF (2006) The genus Thermus and relatives. In: Dworkin M, Falkow S, Rosenberg E, Schleiferand KH, Stackebrandt E (eds) The prokaryotes, 3rd edn, vol 7. Springer, New York, pp 797–812

  9. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    PubMed  Article  CAS  Google Scholar 

  10. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  11. Felsenstein J (2002) PHYLIP (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Science, University of Washington, Seattle, USA

  12. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  13. Hudson JA, Morgan HW, Daniel RM (1987) Thermus filiformis sp. nov., a filamentous caldoactive bacterium. Int J Syst Bacteriol 37:431–436

    Article  Google Scholar 

  14. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol. doi:10.1099/ijs.0.038075-0

  15. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    PubMed  Article  CAS  Google Scholar 

  16. Kristjansson JK, Hjorleifsdottir S, Marteinsson V, Alfredsson GA (1994) Thermus scotoductus, sp. nov., a pigment-producing thermophilic bacterium from hot tap water in Iceland and including Thermus sp. X-1. Syst Appl Microbiol 17:44–50

    Article  Google Scholar 

  17. Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2387

    Article  CAS  Google Scholar 

  18. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428

    PubMed  Article  Google Scholar 

  19. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  20. Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95

    Article  CAS  Google Scholar 

  21. Oshima T, Imahori K (1974) Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. Int J Syst Bacteriol 24:102–112

    Article  CAS  Google Scholar 

  22. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  23. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    PubMed  Article  CAS  Google Scholar 

  24. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    PubMed  Article  CAS  Google Scholar 

  25. Vajna B, Kanizsai S, Kéki Z, Márialigeti K, Schumann P, Tóth EM (2011) Thermus composti sp. nov., isolated from oyster mushroom compost. Int J Syst Evol Microbiol. doi:10.1099/ijs.0.030866-0

  26. Williams RAD, da Costa MS (1992) The genus Thermus and related microorganisms. In: Balows A, Truper HG, Dworkin M, Harder W, Schleifer KH (eds) The prokaryotes, 2nd edn. Springer, New York, p 3745–3753

  27. Williams RAD, Smith KE, Welch SG, Micallef J, Sharp RJ (1995) DNA relatedness of Thermus strains, description of Thermus brockianus sp. nov., and proposal to reestablish Thermus thermophilus (Oshima and Imahori). Int J Syst Bacteriol 45:495–499

    PubMed  Article  CAS  Google Scholar 

  28. Williams RAD, Smith KE, Welch SG, Micallef J (1996) Thermus oshimai sp. nov., isolated from hot springs in Portugal, Iceland, and the Azores, and comment on the concept of a limited geographical distribution of Thermus species. Int J Syst Bacteriol 46:403–408

    PubMed  Article  CAS  Google Scholar 

  29. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu H, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family Oxalobacteraceae isolated from China. Int J Syst Evol Microbiol 55:1149–1153

    PubMed  Article  CAS  Google Scholar 

  30. Zhang XQ, Ying Y, Ye Y, Xu XW, Zhu XF, Wu M (2010) Thermus arciformis sp. nov., a thermophilic species from a geothermal area. Int J Syst Evol Microbiol 60:834–839

    PubMed  Article  CAS  Google Scholar 

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Acknowledgments

The authors are grateful to Prof. Dr. Hans-Peter Klenk for his kind providing reference type strains and Prof. Dr. Iain C. Sutcliffe for his kind help to further polishing this manuscript. This research was supported by the Key Project of International Cooperation of Ministry of Science & Technology (MOST) (No. S2012ZR0069), China and National Natural Science Foundation of China (No. 31070007). W. J. Li was also supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences.

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Correspondence to Wen-Jun Li.

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Tian-Tian Yu and Ji-Cheng Yao have contributed equally to this work.

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Yu, TT., Yao, JC., Ming, H. et al. Thermus tengchongensis sp. nov., isolated from a geothermally heated soil sample in Tengchong, Yunnan, south-west China. Antonie van Leeuwenhoek 103, 513–518 (2013). https://doi.org/10.1007/s10482-012-9833-9

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Keywords

  • Thermophile
  • Thermus tengchongensis sp. nov.
  • 16S rRNA gene
  • Polyphasic taxonomy