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Thermus tengchongensis sp. nov., isolated from a geothermally heated soil sample in Tengchong, Yunnan, south-west China

Antonie van Leeuwenhoek volume 103, pages 513–518 (2013)Cite this article

Abstract

A Gram-stain negative aerobic bacterium, designated YIM 77924T, was isolated from a geothermally heated soil sample collected at Rehai National Park, Tengchong, Yunnan province, south-west China. Growth was found to occur from 55 to 75 °C (optimum 65 °C), pH 6.0–8.0 (optimum pH 7.0) and 0–1 % NaCl (w/v). Cells were observed to be rod-shaped and the colonies convex, circular, smooth, yellow and non-transparent. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM 77924T belongs to the genus Thermus. The 16S rRNA gene sequence similarity values between strain YIM 77924T and other species of the genus Thermus were all below 97 %. The polar lipids of strain YIM 77924T were determined to be aminophospholipid, phospholipid and glycolipid. The predominant respiratory quinone was determined to be MK-8 and the G+C content was 66.64 mol%. The major fatty acids identified were iso-C16:0, iso-C15:0, iso-C17:0 and C16:0. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain YIM 77924T is proposed to represent a novel species, Thermus tengchongensis sp. nov., in the genus Thermus. The type strain is YIM 77924T (=KCTC 32025T = CCTCC AB2012063T).

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Acknowledgments

The authors are grateful to Prof. Dr. Hans-Peter Klenk for his kind providing reference type strains and Prof. Dr. Iain C. Sutcliffe for his kind help to further polishing this manuscript. This research was supported by the Key Project of International Cooperation of Ministry of Science & Technology (MOST) (No. S2012ZR0069), China and National Natural Science Foundation of China (No. 31070007). W. J. Li was also supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences.

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Authors and Affiliations

  1. Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, People’s Republic of China

    Tian-Tian Yu & Wen-Jun Li

  2. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Tian-Tian Yu, Ji-Cheng Yao, Hong Ming, Yi-Rui Yin, En-Min Zhou, Min-Jiao Liu, Shu-Kun Tang & Wen-Jun Li

  3. Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, People’s Republic of China

    Hong Ming

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  1. Tian-Tian Yu
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  2. Ji-Cheng Yao
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Correspondence to Wen-Jun Li.

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Tian-Tian Yu and Ji-Cheng Yao have contributed equally to this work.

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Yu, TT., Yao, JC., Ming, H. et al. Thermus tengchongensis sp. nov., isolated from a geothermally heated soil sample in Tengchong, Yunnan, south-west China. Antonie van Leeuwenhoek 103, 513–518 (2013). https://doi.org/10.1007/s10482-012-9833-9

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Keywords

  • Thermophile
  • Thermus tengchongensis sp. nov.
  • 16S rRNA gene
  • Polyphasic taxonomy