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Halomonas cibimaris sp. nov., isolated from jeotgal, a traditional Korean fermented seafood

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Abstract

Two moderately halophilic, facultatively aerobic, motile bacteria with flagella, designated strains 10-C-3T and 30-C-3, were isolated from jeotgal, a traditional Korean fermented seafood. Cells of the strains were observed to be ovoid-rods showing catalase- and oxidase-positive reactions and production of creamy-pink pigments. Growth of strain 10-C-3T was observed at 15–35 °C (optimum, 25–30 °C), at pH 5.5–9.0 (optimum, pH 7.0–7.5), and in the presence of 3–15 % (w/v) salts (optimum: 5–10 %). The two strains were found to contain C18:1 ω7c, C16:0, summed feature 3 (as defined by the MIDI system, comprising C16:1 ω7c and/or C16:1 ω6c), and C12:0 3-OH as the major cellular fatty acids. The G+C contents of the genomic DNA of strains 10-C-3T and 30-C-3 were determined to be 63.2 and 63.1 mol%, respectively and the respiratory quinone detected was ubiquinone 9 (Q-9) only. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains 10-C-3Tand 30-C-3 formed a distinct phyletic lineage within the genus Halomonas and are most closely related to Halomonas fontilapidosi 5CRT with 95.2 % of 16S rRNA sequence similarity. Strains 10-C-3Tand 30-C-3 shared 99.2 % of 16S rRNA gene sequence similarity and their DNA–DNA relatedness value was 96.6 ± 0.9 %. On the basis of phenotypic, chemotaxonomic and molecular features, strains 10-C-3Tand 30-C-3 represent a novel species of the genus Halomonas, for which the name Halomonas cibimaris sp. nov. is proposed. The type strain is 10-C-3T (= KACC 14932T = JCM 16914T).

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Acknowledgments

These efforts were supported by the Technology Development Program for Agriculture and Forestry (TDPAF) of the Ministry for Agriculture, Forestry and Fisheries and the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment, Republic of Korea.

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Authors and Affiliations

  1. Department of Life Science and Research Center for Biomolecules and Biosystems, Chung-Ang University, 84, HeukSeok-Ro, Dongjak-Gu, Seoul, 156-756, Republic of Korea

    Sang Hyeon Jeong, Ji Young Jung, Se Hee Lee, Moon Su Park & Che Ok Jeon

  2. Department of Food Science and Biotechnology, Kyonggi University, Suwon, 443-760, Republic of Korea

    Jong Hoon Lee

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  1. Sang Hyeon Jeong
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Correspondence to Che Ok Jeon.

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Jeong, S.H., Lee, J.H., Jung, J.Y. et al. Halomonas cibimaris sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Antonie van Leeuwenhoek 103, 503–512 (2013). https://doi.org/10.1007/s10482-012-9832-x

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