A novel plant-associated obligate methylotrophic bacterium, designated strain Ca-68T, was isolated from the rhizosphere soil of field-grown red pepper from India. The isolates are strictly aerobic, Gram negative, motile rods multiplying by binary fission and formaldehyde is assimilated via the ribulose monophosphate pathway. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacillus flagellatus, Methylobacillus glycogens and Methylobacillus pratensis, with which it showed pairwise similarity of 97.8, 97.4 and 96.2 %, respectively. The major fatty acids are C16:0, C10:0 3OH and C16:1 ω7c. The G+C content of the genomic DNA is 59.7 mol%. The major ubiquinone is Q-8. Dominant phospholipids are phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on 16S rRNA gene sequence analysis and DNA–DNA relatedness (14–19 %) with type strains of the genus Methylobacillus, the novel isolate was classified as a new species of this genus and named Methylobacillus rhizosphaerae Ca-68T (=KCTC 22383T = NCIMB 14472T).
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We wish to thank Dr. Peter N. Green, NCIMB, Aberdeen, Scotland, UK for his valuable advice. We also thank Professor Jean P. Euzéby for his valuable advice on the nomenclature. The authors also acknowledge the financial support by grant from the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program, Daejeon, and Republic of Korea.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and mxaF gene sequence of strain Ca-68T is EU672802 and EU912492, respectively.
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Madhaiyan, M., Poonguzhali, S., Senthilkumar, M. et al. Methylobacillus rhizosphaerae sp. nov., a novel plant-associated methylotrophic bacterium isolated from rhizosphere of red pepper. Antonie van Leeuwenhoek 103, 475–484 (2013). https://doi.org/10.1007/s10482-012-9828-6
- Red pepper