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Wangella harbinensis gen. nov., sp. nov., a new member of the family Micromonosporaceae

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Abstract

A novel endophytic actinomycete, designated strain NEAU-J3T, was isolated from soybean root (Glycine max (L.) Merr) and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain NEAU-J3T fell within the family Micromonosporaceae. The strain was observed to form an extensively branched substrate mycelium, which carried non-motile oval spores with a smooth surface. The cell walls of strain NEAU-J3T were determined to contain meso-diaminopimelic acid and galactose, ribose and glucose were detected as whole-cell sugars. The major menaquinones were determined to be MK-9(H4) and MK-9(H6). The phospholipids detected were phosphatidylcholine and phosphatidylethanolamine. The major cellular fatty acids were determined to be C16:0, C18:1 ω9c, C18:0, C17:0, C17:1 ω7c, anteiso-C17:0, C16:1 ω7c and C15:0. The DNA G + C content was 62.5 mol%. On the basis of the morphological and chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain NEAU-J3T is considered to represent a novel species of a new genus within the family Micromonosporaceae, for which the name Wangella harbinensis gen. nov., sp. nov. is proposed. The type strain of Wangella harbinensis is strain NEAU-J3T (=CGMCC 4.7039T = DSM 45747T).

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Acknowledgments

This work was supported in part by grants from NKPBR (No. 2010CB126102) and NNSF (Nos. 30971937 and 30771427) of China, the Special Foundation for Scientific and Technological Innovation Research of Harbin (No. 2011RFXXN038), NSF of Heilongjiang Province (No. C201029).

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  1. School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Feiyu Jia, Chongxi Liu, Xiangjing Wang, Junwei Zhao, Qianfeng Liu, Ji Zhang, Ruixia Gao & Wensheng Xiang

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  1. Feiyu Jia
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  2. Chongxi Liu
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  3. Xiangjing Wang
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Correspondence to Wensheng Xiang.

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Feiyu Jia and Chongxi Liu contributed equally to this work.

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Jia, F., Liu, C., Wang, X. et al. Wangella harbinensis gen. nov., sp. nov., a new member of the family Micromonosporaceae . Antonie van Leeuwenhoek 103, 399–408 (2013). https://doi.org/10.1007/s10482-012-9820-1

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