Paenibacillus hordei sp. nov., isolated from naked barley in Korea

Abstract

A Gram-staining positive, facultative aerobic bacterium, designated strain RH-N24T, was isolated from naked barley in South Korea. Cells of the isolate were observed to be motile rods by means of peritrichous flagella and showed catalase-positive and oxidase-negative reactions. Growth of strain RH-N24T was observed at 4–40 °C (optimum: 35–37 °C) and at pH 5.0–9.0 (optimum: pH 6.0–7.0). Chemotaxonomic data (major isoprenoid quinone: MK-7; DNA G + C content: 53.5 mol %; cell wall type: A1γ-meso-diaminopimelic acid; major fatty acids: anteiso-CB15:0 and CB16:0B) supported the affiliation of the isolate to the genus Paenibacillus. The major cellular polar lipids were identified as phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and two unidentified polar lipids. Phylogenetic analysis based on 16S rRNA gene sequences also supported the conclusion that strain RH-N24T belonged to the genus Paenibacillus. Comparative 16S rRNA gene sequence analysis showed that strain RH-N24T was most closely related to Paenibacillus hunanensis FeL05T and Paenibacillus illinoisensis NRRL NRS-1356T with similarities of 94.64 and 94.54 %, respectively. On the basis of phenotypic and molecular properties, strain RH-N24T represents a novel species within the genus Paenibacillus for which the name Paenibacillus hordei sp. nov. is proposed. The type strain is RH-N24T (=KACC 15511T = JCM 17570T).

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Acknowledgments

These efforts were supported by the Technology Development Program for Agriculture and Forestry (TDPAF) of the Ministry for Agriculture, Forestry and Fisheries and the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment, Republic of Korea. The GenBank accession number for the 16S rRNA gene sequence of strain RH-N24T is HQ833590.

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Correspondence to Che Ok Jeon.

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Kim, J.M., Lee, S.H., Lee, S.H. et al. Paenibacillus hordei sp. nov., isolated from naked barley in Korea. Antonie van Leeuwenhoek 103, 3–9 (2013). https://doi.org/10.1007/s10482-012-9775-2

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Keywords

  • Paenibacillus hordei
  • Novel species
  • Firmicutes
  • Gram-positive bacteria
  • Naked barley