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Lihuaxuella thermophila gen. nov., sp. nov., isolated from a geothermal soil sample in Tengchong, Yunnan, south-west China

Antonie van Leeuwenhoek volume 102, pages 711–718 (2012)Cite this article

Abstract

A novel filamentous bacterium, designated YIM 77831T, was isolated from a geothermal soil sample collected at Rehai National Park, Tengchong, Yunnan province, south-west China. Growth occurred from 28 to 65 °C (optimum 50 °C), pH 6.0–8.0 (optimum pH 7.0). The strain formed branched substrate mycelia, endospores were produced on the substrate mycelium and aerial mycelium was not produced on any of the growth media tested. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM 77831T was affiliated with the family Thermoactinomycetaceae. The stain YIM 77831T contained meso-diaminopimelic acid in the cell wall. Whole-cell hydrolysates contained glucose, galactose, mannose, ribose and rhamnose. The polar lipids were phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid and four unknown phospholipids. The only menaquinone was MK-7. Major fatty acids were iso-C15:0, anteiso-C15:0 and anteiso-C17:0. The G+C content was 55.6 mol%. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain YIM 77831T represents a novel genus and species, Lihuaxuella thermophila gen. nov., sp. nov., in the family Thermoactinomycetaceae. The type strain is YIM 77831T (CCTCC AA 2011024T = JCM 18059T).

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Acknowledgments

The authors are grateful to Prof. J.P. Euzeby for his kind help to check and correct our Latin etymology for the genus and species name. This research was supported by National Natural Science Foundation of China (No. 31070007), International Cooperation Research Program of Yunnan Province (No. 2009AC017) and Yunnan Provincial Sciences and Technology Department (No. 2011FB034). W-J Li was also supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences.

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Affiliations

  1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Tian-Tian Yu, Bing-Huo Zhang, Ji-Cheng Yao, Shu-Kun Tang, En-Min Zhou, Yi-Rui Yin & Wen-Jun Li

  2. College of Life Sciences, Jiujiang University, Jiujiang, 332000, People’s Republic of China

    Bing-Huo Zhang

  3. Faculty of Environmental Science and Engineering & Life Science and Technology, Kunming University of Science and Technology, Kunming, 650224, People’s Republic of China

    Da-Qiao Wei

  4. Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, People’s Republic of China

    Hong Ming

  5. Key Laboratory of Biogeography and Bioresource in Arid Land, Chinese Academy of Science, Xinjiang Institute of Ecology and Geography, Ürűmqi, 830011, People’s Republic of China

    Wen-Jun Li

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  1. Tian-Tian Yu
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Yu, TT., Zhang, BH., Yao, JC. et al. Lihuaxuella thermophila gen. nov., sp. nov., isolated from a geothermal soil sample in Tengchong, Yunnan, south-west China. Antonie van Leeuwenhoek 102, 711–718 (2012). https://doi.org/10.1007/s10482-012-9771-6

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Keywords

  • Lihuaxuella thermophila gen. nov., sp. nov.
  • Polyphasic taxonomy
  • Rehai National Park