Pseudonocardia nantongensis sp. nov., a novel endophytic actinomycete isolated from the coastal halophyte Tamarix chinensis Lour

Abstract

A novel isolate, designated strain KLBMP 1282T was isolated from the surface-sterilized leaves of a coastal halophyte Tamarix chinensis Lour., collected from Nantong, Jiangsu Province, east of China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this strain belongs to the genus Pseudonocardia, being most closely related to Pseudonocardia kongjuensis LM 157T (98.33 %), Pseudonocardia autotrophica IMSNU 20050T (97.77 %), Pseudonocardia endophytica YIM 56035T (97.63 %), Pseudonocardia ammonioxydans H9 T (97.62 %) and Pseudonocardia compacta IMSNU 20111T (97.56 %); similarity to other type strains of the genus Pseudonocardia was <97.5 %. Chemotaxonomic data confirmed the affiliation of strain KLBMP 1282T to the genus Pseudonocardia. Strain KLBMP 1282T contained MK-8(H4) as the predominant ubiquinone and iso-C16:0 as the major fatty acid. The polar lipids detected in strain KLBMP 1282T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, one unknown phospholipid and four unknown glycolipids. The DNA G + C content of strain KLBMP 1282T was 73.1 mol %. The results of DNA–DNA hybridizations and the phylogenetic analysis, together with the phenotypic and biochemical tests, allowed the differentiation of strain KLBMP 1282T from strains of other recognized Pseudonocardia species. Therefore, strain KLBMP 1282T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia nantongensis sp. nov. is proposed. The type strain is KLBMP 1282T (=KCTC 29053T = NBRC 108677T).

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Acknowledgments

The authors are grateful to Prof. Tomohiko Tamura (NITE Biological Resource Center, NBRC) for kindly providing the type strains and Prof. Martha E. Trujillo for his valuable comments on the manuscript. This research was partially supported by National Natural Science Foundation of China (31000005, 31101502), the Program of Natural Science Foundation of the Jiangsu Higher Education Institutions of China (10KJB180008, 11KJD210002), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and Natural Science Foundation by Xuzhou City (No. XZZD1004).

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Correspondence to Sheng Qin or Ji-Hong Jiang.

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Xing, K., Qin, S., Bian, GK. et al. Pseudonocardia nantongensis sp. nov., a novel endophytic actinomycete isolated from the coastal halophyte Tamarix chinensis Lour. Antonie van Leeuwenhoek 102, 659–667 (2012). https://doi.org/10.1007/s10482-012-9764-5

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Keywords

  • Pseudonocardia nantongensis sp. nov
  • Coastal halophyte
  • Endophytic
  • Polyphasic taxonomy