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Lysobacter thermophilus sp. nov., isolated from a geothermal soil sample in Tengchong, south-west China

Antonie van Leeuwenhoek volume 102, pages 643–651 (2012)Cite this article

Abstract

A Gram-negative and aerobic bacterium, designated YIM 77875T, was isolated from a geothermal soil sample collected at Rehai National Park, Tengchong, Yunnan Province, south-west China. Bacterial growth occurred from 37 to 65 °C (optimum 50 °C), pH 6.0–8.0 (optimum pH 7.0) and 0–1 % NaCl (w/v). Cells were rod-shaped and colonies were convex, circular, smooth, yellow and non-transparent. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM 77875T belongs to the genus Lysobacter. The 16S rRNA gene sequence similarity values between strain YIM 77875T and other species of the genus Lysobacter were all below 94.7 %. The polar lipids of strain YIM 77875T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and five unknown phospholipids. The predominant respiratory quinone was Q-8 and the G+C content was 68.8 mol%. Major fatty acids were iso-C16:0, iso-C15:0 and iso-C11:0. On the basis of the morphological and chemotaxonomic characteristics, as well as genotypic data, strain YIM 77875T represents a novel species, Lysobacter thermophilus sp. nov., in the genus Lysobacter. The type strain is YIM 77875T (CCTCC AB 2012064T = KCTC 32020T).

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Acknowledgments

We are grateful to Prof. Cheng-Xiang Fang (CCTCC, China) for his kind providing reference type strain L. xinjiangensis CCTCC AB 208194T. This research was supported by Yunnan Provincial Sciences and Technology Department (No. 2011FB034). W-J Li was also supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences.

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Affiliations

  1. Faculty of Environmental Science and Engineering & Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, People’s Republic of China

    Da-Qiao Wei

  2. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Tian-Tian Yu, Ji-Cheng Yao, En-Min Zhou, Yi-Rui Yin, Shu-Kun Tang & Wen-Jun Li

  3. Engineering Technology Research Center of Biomass Degradation and Gas-forming, Shangqiu Normal University, Shangqiu, 476000, People’s Republic of China

    Zhao-Qi Song

  4. Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, People’s Republic of China

    Hong Ming

  5. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, People’s Republic of China

    Wen-Jun Li

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  1. Da-Qiao Wei
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  2. Tian-Tian Yu
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Correspondence to Wen-Jun Li.

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Da-Qiao Wei and Tian-Tian Yu contributed equally to this study.

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Wei, DQ., Yu, TT., Yao, JC. et al. Lysobacter thermophilus sp. nov., isolated from a geothermal soil sample in Tengchong, south-west China. Antonie van Leeuwenhoek 102, 643–651 (2012). https://doi.org/10.1007/s10482-012-9761-8

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Keywords

  • Tengchong
  • Lysobacter thermophilus sp. nov.
  • 16S rRNA gene
  • Polyphasic taxonomy