Kineococcus endophytica sp. nov., a novel endophytic actinomycete isolated from a coastal halophyte in Jiangsu, China

Abstract

A novel Gram-positive, motile, non-spore-forming coccus-shaped bacterial strain, designated KLBMP 1274T, was isolated from a halophytic plant (Limonium sinense) collected from the coastal region of Nantong, Jiangsu Province, in east China. Phylogenetic analyses based on the 16S rRNA gene sequence showed that strain KLBMP 1274T belongs to the genus Kineococcus and is closely related to Kineococcus rhizosphaerae RP-B16T (98.72 %), Kineococcus aurantiacus IFO 15268T (98.71 %), Kineococcus radiotolerans SRS30216T (98.69 %) and Kineococcus gynurae KKD096T (97.33 %). The 16S rRNA gene sequence similarity to other species of the genus Kineococcus was <97 %. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid, with arabinose and galactose as the characteristic sugars. The predominant menaquinone was MK-9(H2). The polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unknown phospholipid, an unknown glycolipid, and three unknown lipids. Major cellular fatty acids were found to be anteiso-C15: 0 and iso-C14: 0. The chemotaxonomic data for strain KLBMP 1274T were typical of the genus Kineococcus. The total DNA G+C content was 73.4 mol %. DNA–DNA relatedness and differential phenotypic data demonstrated that strain KLBMP 1274T was clearly distinguished from all closely related species of the genus Kineococcus. Thus, strain KLBMP 1274T represents a novel species of the genus Kineococcus, for which the name Kineococcus endophytica sp. nov. is proposed. The type strain is KLBMP 1274T (=KCTC 19886 T = NBRC 108674T).

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Acknowledgments

The authors are grateful to Prof. Tomohiko Tamura (NITE Biological Resource Center, NBRC) for kindly providing the type strains and Prof. Iain C. Sutcliffe for his valuable comments on the manuscript. This research was partially supported by National Natural Science Foundation of China (31000005, 31101502), the Program of Natural Science Foundation of the Jiangsu Higher Education Institutions (10KJB180008, 11KJD210002), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and Natural Science Foundations by Xuzhou City (No. XZZD1004).

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Correspondence to Sheng Qin or Ji-Hong Jiang.

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Guang-Kai Bian and Zhao-Zhong Feng contributed equally to this work.

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Bian, GK., Feng, ZZ., Qin, S. et al. Kineococcus endophytica sp. nov., a novel endophytic actinomycete isolated from a coastal halophyte in Jiangsu, China. Antonie van Leeuwenhoek 102, 621–628 (2012). https://doi.org/10.1007/s10482-012-9757-4

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Keywords

  • Kineococcus endophytica sp. nov.
  • 16S rRNA gene
  • Endophytic actinomycete
  • Polyphasic taxonomy