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Streptomyces oceani sp. nov., a new obligate marine actinomycete isolated from a deep-sea sample of seep authigenic carbonate nodule in South China Sea

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Abstract

A novel aerobic actinomycete strain, designated as SCSIO 02100T, was isolated from a deep sea sediment sample collected from Northern South China Sea at a depth of 578 m. This isolate requires sea water or a sodium-supplemented medium for growth. BLAST searches based on the almost full length of the 16S rRNA gene sequence, showed that strain SCSIO 02100T had the highest similarities with Streptomyces armeniacus (JCM 3070T) (97.1 %). Phylogenetic trees reconstructed on the basis of 16S rRNA gene sequences revealed that strain SCSIO 02100T formed a distinct lineage with S. nanshensis SCSIO 01066T with 96.9 % similarity. Further analysis of the polyphasic taxonomic data, including morphological, phenotypic and chemotaxonomic properties, showed that strain SCSIO 02100T could be readily distinguished from the most closely related members of the genus Streptomyces. Thus, based on the polyphasic taxonomic data, a novel species, Streptomyces oceani sp. nov., is proposed, with the type strain SCSIO 02100T (=DSM 42043T = CGMCC 4.7007T).

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Acknowledgments

This research was supported by the National Basic Research Program of China (2010CB833801), Knowledge Innovation Program of Chinese Academy of Sciences (KSCX2-YW-G-065, KSCX2-EW-G-12, KZCX2-YW-JC202), the National Natural Science Foundation of China (No. 40906075), and the 21C Frontier Program of Microbial Genomics and Application from the MEST, Korea. South China Sea Open Cruise by R/V Shiyan 3, South China Sea Institute of Oceanology, CAS. Also thanks to Prof. Zhang Lü-Ping in South China Sea institute of Oceanology to observe the micromorphology of this strain by using scanning electron microscopy.

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  1. Key Laboratory of Marine Bio-resources Sustainable Utilization, CAS, RNAM Center for Marine Microbiology, CAS, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People’s Republic of China

    Xin-Peng Tian, Jie Li, Chang-Sheng Zhang & Si Zhang

  2. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Ying Xu, Jing Zhang & Wen-Jun Li

  3. Key Laboratory of Marginal Sea Geology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Zhong Chen

  4. Korea Research Institutes of Biosciences and Biotechnology, 52 Eoeun-dong, Yuseong gu, Daejeon, 305-333, Republic of Korea

    Chang-Jin Kim

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  1. Xin-Peng Tian
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Correspondence to Si Zhang.

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Tian, XP., Xu, Y., Zhang, J. et al. Streptomyces oceani sp. nov., a new obligate marine actinomycete isolated from a deep-sea sample of seep authigenic carbonate nodule in South China Sea. Antonie van Leeuwenhoek 102, 335–343 (2012). https://doi.org/10.1007/s10482-012-9743-x

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