Abstract
A Gram-negative, coccoid shaped bacterium isolated from the outer surface of the medicinal leech Hirudo medicinalis was characterized. The 16S rRNA gene sequence comparison revealed that the bacterium was closely related to species of the genus Luteolibacter. Luteolibacter pohnpeiensis was the most closely related species (94.6 % sequence similarity), followed by Luteolibacter luojiensis (93.4 %) and Luteolibacter algae (93.3 %). Chemotaxonomic data (major ubiquinone: MK-9; major polar lipids: phosphatidylethanolamine and phosphatidylglycerol; and major fatty acids: iso-C14:0, C16:0, iso-C16:1, and anteiso-C15:0) supported the affiliation of the isolate to the genus Luteolibacter. DNA–DNA hybridizations with the type strain of L. pohnpeiensis was 31 % (reciprocal value 30 %). A phenotypic differentiation of strain E100T from L. pohnpeiensis and the other Luteolibacter species was possible by several physiological tests. We conclude Strain E100T represents a novel species, for which we propose the name Luteolibacter cuticulihirudinis sp. nov. with the type strain E100T (=CCM 8400T = LMG 26924T).
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We thank Gundula Will, Carmen Schult, Maria Sowinsky and Anna Baum for excellent technical assistance and Dr. Jean Euzéby for his nomenclatural advice.
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Supplementary Fig. 1: Neighbor-joining tree showing the phylogenetic position of strain E100T within the Verrucomicrobiaceae. The tree was generated in ARB using ARB neighbor-joining (Jukes-Cantor, 100 bootstraps) and based on 16S rRNA gene sequences between positions 57 and 1454 (E. coli numbering; Brosius et al. 1978). GenBank accession numbers are given in parentheses. Numbers at branch nodes refer to bootstrap values >70 % (100 replicates). Victivallis vadensis CelloT and Lentisphaera araneosa HTCC 2155T were used as outgroups. Bar 0.10 substitutions per side
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Glaeser, S.P., Galatis, H., Martin, K. et al. Luteolibacter cuticulihirudinis sp. nov., isolated from Hirudo medicinalis . Antonie van Leeuwenhoek 102, 319–324 (2012). https://doi.org/10.1007/s10482-012-9741-z
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DOI: https://doi.org/10.1007/s10482-012-9741-z