Abstract
Two strains of a basidiomycetous yeast were derived from an insect trypanosomatid culture isolated from the intestine of a plant bug, Collaria oleosa (Heteroptera: Miridae), collected in Costa Rica. The yeast did not form ballistoconidia but reproduced only by budding. Teliospores were not observed in individual and crossed cultures of each strain. Morphological and other taxonomic characteristics of the yeast were similar to those of the species in the polyphyletic genus Rhodotorula. However, molecular phylogeny inferred from the internal transcribed spacers and D1/D2 region of the large subunit rRNA gene showed that the strains represent a new species placed among the smut fungi in the family Ustilentylomataceae, which includes Aurantiosporium subnitens, Fulvisporium restifaciens, Ustilentyloma fluitans, and Rhodotorula hordea. Given the well distinguished phylogenetic position of this novel species within the Ustilentylomataceae, we propose Microbotryozyma collariae gen. nov., sp. nov. to accommodate the yeast isolated from C. oleosa, with strain American Type Culture Collection MYA-4666T (= PRA303-1S = CBS 12537) designated as the type strain.
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Acknowledgments
We thank Drs. Brian Beck and Dominik Begerow for their useful comments during this study, and Neil Hetrick, ATCC Protistology Collection, for his assistance in the culture of Leptomonas barvae (ATCC PRA-303). We are also grateful to Pushpa Gujjari and Janice L. Houseknecht, ATCC Mycology Collection, for their assistance in characterizing the yeasts. Many thanks to the two anonymous reviewers for their valuable comments, especially on proposing a novel genus in this manuscript.
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The GenBank accession numbers for the ribosomal RNA gene sequences determined in this study are JN849458 to JN849461.
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Suh, SO., Maslov, D.A., Molestina, R.E. et al. Microbotryozyma collariae gen. nov., sp. nov., a basidiomycetous yeast isolated from a plant bug Collaria oleosa (Miridae). Antonie van Leeuwenhoek 102, 99–104 (2012). https://doi.org/10.1007/s10482-012-9717-z
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DOI: https://doi.org/10.1007/s10482-012-9717-z