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Amycolatopsis bartoniae sp. nov. and Amycolatopsis bullii sp. nov., mesophilic actinomycetes isolated from arid Australian soils

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Abstract

The status of two mesophilic filamentous actinomycetes isolated from an arid Australian soil sample was determined using a polyphasic taxonomic approach. The isolates had chemical and morphological properties consistent with their classification in the genus Amycolatopsis, assignments that were supported by analysis of 16S rRNA gene sequence data. Isolate SF26T formed a distinct phyletic line and hence was sharply separated from its nearest phylogenetic neighbour, Amycolatopsis sacchari DSM 44468T. In contrast, isolate SF27T formed a subclade in the Amycolatopsis tree with Amycolatopsis vancoresmycina DSM 44592T but was separated readily from the latter by DNA:DNA pairing data. The two isolates were distinguished from one another and from their respective nearest phylogenetic neighbours using a range of phenotypic properties. These data indicate that the two isolates should be recognized as new species in the genus Amycolatopsis. The names proposed for these new taxa are Amycolatopsis bartoniae sp. nov. and Amycolatopsis bullii sp. nov. with isolates SF26T (=NCIMB 14706T = NRRL B-2846T) and SF27T (=NCIMB 14707T = NRRL B-24847T) as the respective type strains.

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Acknowledgments

TDZ is indebted to the ConselhoNacional de DesenvolvimentoCientífico e Tecnológico for a fellowship to study in the UK (Grant 201066/2009-2). We are grateful to Professor Jean Euzéby (EcoléNationaleVétérinaire de Toulouse, France) for help in naming the strains. The GenBank accession numbers for the 16S rRNA gene sequences of Amycolatopsis bartoniae SF26T and Amycolatopsis bullii SF27T are HQ651729 and HQ651730, respectively.

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Authors and Affiliations

  1. School of Biology, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK

    Tiago Domingues Zucchi, Avinash Naga Venkata Bonda, Sarah Frank, Byung-Yong Kim, Jenileima Devi Kshetrimayum & Michael Goodfellow

  2. Laboratório de Microbiologia Ambiental, EMBRAPA Meio Ambiente, Jaguariúna, 13820-000, Brazil

    Tiago Domingues Zucchi

  3. Department of Agricultural Microbiology, National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Republic of Korea

    Byung-Yong Kim

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  1. Tiago Domingues Zucchi
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  2. Avinash Naga Venkata Bonda
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Correspondence to Michael Goodfellow.

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Zucchi, T.D., Bonda, A.N.V., Frank, S. et al. Amycolatopsis bartoniae sp. nov. and Amycolatopsis bullii sp. nov., mesophilic actinomycetes isolated from arid Australian soils. Antonie van Leeuwenhoek 102, 91–98 (2012). https://doi.org/10.1007/s10482-012-9716-0

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