Abstract
The present study describes the in vivo ameliorating effect of Lactobacillus casei supplementation in renourished Giardia intestinalis infected BALB/c mice. It was observed that daily administration of probiotic 7 days prior to Giardia-infection to renourished mice, efficiently reduced the excretion of Giardia cysts and trophozoite counts, along with significant increased fecal lactobacilli counts compared with Giardia-infected mice. It was also observed that oral feeding of probiotic to renourished-Giardia-infected mice abrogated all the anthropometric and biochemical anomalies. Histologically, morphological and cellular alteration of microvillus membrane integrity revealed that probiotic administration further ameliorated the mucosal damage in renourished-probiotic-Giardia-infected mice compared to severe microvillus atrophy, oedematous, vacuolated epithelial cells and ileitis in renourished-Giardia and Giardia-infected mice. Thus, it is suggested that probiotic used as the functional food helps in restoration of anthropometric, biochemical alterations and atrophied gut by enhancing the goblet cells and reducing the giardiasis.
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Acknowledgments
Authors are thankful to Mr. Bhandari, Senior Technician, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh India, for maintaining and providing the Giardia intestinalis (Portland I) culture. Financial assistance provided by UGC/SAP to carry out the present study is highly acknowledged.
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Shukla, G., Sidhu, R.K. & Verma, A. Restoration of anthropometric, biochemical and histopathological alterations by Lactobacillus casei supplementation in Giardia intestinalis infected renourished BALB/c mice. Antonie van Leeuwenhoek 102, 61–72 (2012). https://doi.org/10.1007/s10482-012-9713-3
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DOI: https://doi.org/10.1007/s10482-012-9713-3