Skip to main content
SpringerLink
Log in

Candida wangnamkhiaoensis sp. nov., an anamorphic yeast species in the Hyphopichia clade isolated in Thailand

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

Two strains representing a single novel yeast species were isolated from a flower of Calycoopteris floribunda Lame (SK170T) and insect frass (ST-122) collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer region, the two strains were assigned as a single novel Candida species in the Hyphopichia clade for which the name Candida wangnamkhiaoensis sp. nov. is proposed. The type strain is SK170T=BCC 39604T=NBRC 106724T=CBS 11695T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Altschul SF, Madden TL, Schäffer JZ, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Groenewald M, Smith MT (2010) Re-examination of strains formerly assigned to Hyphopichia burtonii, the phylogeny of the genus Hyphopichia, and the description of Hyphopichia pseudoburtonii sp. nov. Int J Syst Evol Microbiol 60:2675–2680

    Article  PubMed  CAS  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  • Kuraishi H, Katayama-Fujimura Y, Sugiyama J, Yokoyama T (1985) Ubiquinone systems in fungi. I. Distribution of ubiquinones in the major families of ascomycetes, basidiomycetes, and deuteromycetes, and their taxonomic implications. Trans Mycol Soc Jpn 26:383–395

    Google Scholar 

  • Kurtzman CP (1998) Pichia E.C. Hasen emend. Kurtzman. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. The Netherlands, Elsevier, pp 273–352

    Chapter  Google Scholar 

  • Kurtzman CP (2005) New species and a new combination in the Hyphopichia and Yarrowia yeast clades. Antonie van Leewenhoek 88:121–130

    Article  CAS  Google Scholar 

  • Kurtzman CP (2011) Hyphopichia von Arx & van der Walt. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts, a taxonomic study, 5th edn, vol 2. Elsevier, Amsterdam, pp 435–438

  • Limtong S, Yongmanitchai W, Tun MM, Kawasaki H, Seki T (2007) Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. Int J Syst Evol Microbiol 57:419–422

    Article  PubMed  Google Scholar 

  • Nagatsuka Y, Kawasaki H, Mikata K, Seki T (2005) Candida khmerensis sp. nov., a novel cation-tolerant yeast isolated from dry salted shrimp and sewage in Cambodia. J Gen Appl Microbiol 51:235–243

    Article  PubMed  CAS  Google Scholar 

  • Nakase T, Suzuki M (1986) Bullera megalospora, a new species of yeast forming larger ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinesis and Sasa sp. in Japan. J Gen Appl Microbiol 32:225–240

    Article  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics (MEGA) Soft-ware version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • von Arx JA, van der Walt JP (1976) The ascigerous state of Candida chodatii. Antonie van Leewenhoek 42:309–314

    Article  CAS  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322

    Google Scholar 

  • Yamada Y, Kondo K (1973) Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus, and the yeast-like genera Sporobolomyces and Rhodosporidium. J Gen Appl Microbiol 19:59–77

    Article  CAS  Google Scholar 

  • Yarrow D (1998) Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 77–100

    Chapter  Google Scholar 

Download references

Acknowledgment

This study was partial supported by the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission.

Author information

Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand

    Savitree Limtong, Rungluk Kaewwichian & Wichien Yongmanitchai

  2. Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Kasetsart University, Bangkok, 10900, Thailand

    Savitree Limtong

  3. Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), Klong Luang, Pathumthani, 12120, Thailand

    Sasitorn Jindamorakot & Takashi Nakase

Authors
  1. Savitree Limtong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rungluk Kaewwichian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sasitorn Jindamorakot
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wichien Yongmanitchai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takashi Nakase
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Savitree Limtong.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Limtong, S., Kaewwichian, R., Jindamorakot, S. et al. Candida wangnamkhiaoensis sp. nov., an anamorphic yeast species in the Hyphopichia clade isolated in Thailand. Antonie van Leeuwenhoek 102, 23–28 (2012). https://doi.org/10.1007/s10482-012-9709-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-012-9709-z

Keywords

Search

Navigation