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Verrucosispora wenchangensis sp. nov., isolated from mangrove soil

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Abstract

An actinomycete strain 234402T was isolated from a mangrove soil sample collected in Wenchang, China. Phylogenetic analysis of the 16S rRNA gene sequence of strain 234402T indicated that the highest similarity was to Verrucosispora sediminis MS426T (99.25%). The cell wall contained meso-diaminopimelic acid. The major menaquinones were MK-9(H4) and MK-9(H6), with MK-9(H8) as minor components. The characteristic whole-cell sugars were xylose, mannose and glucose. The phospholipid profile was found to comprise phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside and an unknown phospholipid. The DNA G+C content was 69.2 mol%. The results of physiological and biochemical tests and low DNA–DNA relatedness demonstrated strain 234402T could be readily distinguished from the closely related Verrucosispora species. On the basis of these phenotypic and genotypic data, strain 234402T represents a novel species of the genus Verrucosispora, for which the name Verrucosispora wenchangensis sp. nov. is proposed. The type strain is 234402T (=CCTCC AA 2011018T=DSM 45674T).

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Acknowledgment

This investigation was co-supported by National Natural Science Foundation of China (31170467), and National Nonprofit Institute Research Grant of CATAS-ITBB (ITBBZD0753). The authors thank Mr Wenquan Li for his help on sample collection.

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Authors and Affiliations

  1. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, People’s Republic of China

    Lei Li, Zixin Deng & Kui Hong

  2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, People’s Republic of China

    Qing-yi Xie, Hai-peng Lin, Lei Li & Kui Hong

  3. School of Biology, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK

    Roselyn Brown & Michael Goodfellow

Authors
  1. Qing-yi Xie
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  2. Hai-peng Lin
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  3. Lei Li
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  4. Roselyn Brown
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  5. Michael Goodfellow
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  6. Zixin Deng
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  7. Kui Hong
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Correspondence to Kui Hong.

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Xie, Qy., Lin, Hp., Li, L. et al. Verrucosispora wenchangensis sp. nov., isolated from mangrove soil. Antonie van Leeuwenhoek 102, 1–7 (2012). https://doi.org/10.1007/s10482-012-9707-1

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  • DOI: https://doi.org/10.1007/s10482-012-9707-1

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