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Antonie van Leeuwenhoek

, Volume 101, Issue 4, pp 787–794 | Cite as

Oleispira lenta sp. nov., a novel marine bacterium isolated from Yellow sea coastal seawater in Qingdao, China

  • Yan Wang
  • Min Yu
  • Brian Austin
  • Xiao-Hua Zhang
Original Paper

Abstract

The taxonomic position of strain DFH11T, which was isolated from coastal seawater off Qingdao, People’s Republic of China in 2007, was determined. Strain DFH11T comprised Gram-negative, motile, strictly aerobic spirilli that did not produce catalase. Comparative 16S rRNA gene sequence analysis revealed that strain DFH11T shared ~97.2, 93.3, 91.8, 91.7 and 91.5% sequence similarities with Oleispira antarctica, Spongiispira norvegica, Bermanella marisrubri, Oceaniserpentilla haliotis and Reinekea aestuarii, respectively. DNA–DNA hybridization experiments indicated that the strain was distinct from its closest phylogenetic neighbour, O. antarctica. The strain grew optimally in 2–3% (w/v) NaCl, at pH 5.0–10.0 (optimally at pH 7.0) and between 0 and 30°C (optimum growth temperature 28°C). The strain exhibited a restricted substrate profile, with a preference for aliphatic hydrocarbons, that is consistent with its closest phylogenetic neighbour O. antarctica. Growth of the isolate at different temperatures affected the cellular fatty acid profile. 28°C cultured cells contained C16:1ω7c and/or iso-C15:0 2-OH (50.4%) and C16:0 (19.2%) as the major fatty acids. However, the major fatty acids of the cells cultured at 4°C were C16:1ω7c and/or C16:1ω6c (40.2%), C16:0 (17.2%) and C17:1ω8c (10.1%). The G+C content of the genomic DNA was 42.7 mol%. Phylogeny based on 16S rRNA gene sequences together with data from DNA–DNA hybridization, phenotypic and chemotaxonomic characterization revealed that DFH11T should be classified as a novel species of the genus Oleispira, for which the name Oleispira lenta sp. nov. is proposed, with the type strain DFH11T (=NCIMB 14529T = LMG 24829T).

Keywords

Oleispiralenta sp. nov. Taxonomy Yellow sea 

Notes

Acknowledgments

This work was supported by a grant from the National High Technology Research, Development Program of China (863 Program, No. 2007AA09Z434) and a grant from the National Natural Science Foundation of China (No. 40876067).

Supplementary material

10482_2011_9693_MOESM1_ESM.doc (28 kb)
Supplementary material 1 (DOC 28 kb)
10482_2011_9693_MOESM2_ESM.tif (9 mb)
Supplementary material 2 (TIFF 9,242 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Yan Wang
    • 1
    • 2
  • Min Yu
    • 1
  • Brian Austin
    • 3
  • Xiao-Hua Zhang
    • 1
  1. 1.College of Marine Life SciencesOcean University of ChinaQingdaoPeople’s Republic of China
  2. 2.Department of Life Sciences and TechnologyXinxiang Medical UniversityXinxiangPeople’s Republic of China
  3. 3.Institute of AquacultureUniversity of StirlingStirlingUK

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