Oleispira lenta sp. nov., a novel marine bacterium isolated from Yellow sea coastal seawater in Qingdao, China
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The taxonomic position of strain DFH11T, which was isolated from coastal seawater off Qingdao, People’s Republic of China in 2007, was determined. Strain DFH11T comprised Gram-negative, motile, strictly aerobic spirilli that did not produce catalase. Comparative 16S rRNA gene sequence analysis revealed that strain DFH11T shared ~97.2, 93.3, 91.8, 91.7 and 91.5% sequence similarities with Oleispira antarctica, Spongiispira norvegica, Bermanella marisrubri, Oceaniserpentilla haliotis and Reinekea aestuarii, respectively. DNA–DNA hybridization experiments indicated that the strain was distinct from its closest phylogenetic neighbour, O. antarctica. The strain grew optimally in 2–3% (w/v) NaCl, at pH 5.0–10.0 (optimally at pH 7.0) and between 0 and 30°C (optimum growth temperature 28°C). The strain exhibited a restricted substrate profile, with a preference for aliphatic hydrocarbons, that is consistent with its closest phylogenetic neighbour O. antarctica. Growth of the isolate at different temperatures affected the cellular fatty acid profile. 28°C cultured cells contained C16:1ω7c and/or iso-C15:0 2-OH (50.4%) and C16:0 (19.2%) as the major fatty acids. However, the major fatty acids of the cells cultured at 4°C were C16:1ω7c and/or C16:1ω6c (40.2%), C16:0 (17.2%) and C17:1ω8c (10.1%). The G+C content of the genomic DNA was 42.7 mol%. Phylogeny based on 16S rRNA gene sequences together with data from DNA–DNA hybridization, phenotypic and chemotaxonomic characterization revealed that DFH11T should be classified as a novel species of the genus Oleispira, for which the name Oleispira lenta sp. nov. is proposed, with the type strain DFH11T (=NCIMB 14529T = LMG 24829T).
KeywordsOleispiralenta sp. nov. Taxonomy Yellow sea
This work was supported by a grant from the National High Technology Research, Development Program of China (863 Program, No. 2007AA09Z434) and a grant from the National Natural Science Foundation of China (No. 40876067).
- Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) (1995) Short protocols in molecular biology: a compendium of methods from current protocols in molecular biology, 3rd edn. Wiley, New YorkGoogle Scholar
- Cowan ST, Steel KJ (1974) Cowan and Steel’s manual for the identification of medical bacteria, 2nd edn. Revised by Cowan ST. Cambridge University Press, CambridgeGoogle Scholar
- Garrity GM (2005) The proteobacteria. In: Krieg NR, Boone DR, Castenholz RW, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol 2, 2nd edn. Springer-Verlag, New YorkGoogle Scholar
- Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) (1994) Methods for general and molecular bacteriology. America Society for Microbiology, Washington DCGoogle Scholar
- Satomi M, Kimura B, Hamada T, Harayama S, Fujii T (2002) Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum, description of Pseudospirillum gen. nov. Oceanobacter gen. nov. and Terasakiella gen. nov. and transfer of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. nov. Int J Syst Evol Microbiol 52:739–747PubMedCrossRefGoogle Scholar
- Yakimov MM, Giuliano L, Gentile G, Crisafi E, Chernikova TN, Abraham WR, Lünsdorf H, Timmis KN, Golyshin PN (2003) Oleispira antarctica gen. nov. sp. nov. a novel hydrocarbonoclastic marine bacterium isolated from Antarctic coastal sea water. Int J Syst Evol Microbiol 53:779–785PubMedCrossRefGoogle Scholar