Cohnella boryungensis sp. nov., isolated from soil

Abstract

A Gram-staining positive, endospore-forming, motile and rod-shaped bacterial strain, BR-29T, was isolated from soil from west coast of the Korean peninsula, and its taxonomic position was investigated by a polyphasic study. Strain BR-29T grew optimally at around pH 7.5, at 30°C and in the presence of 0.5% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BR-29T fell into a clade comprising the type strains of Cohnella species, with which it exhibited 16S rRNA gene sequence similarity values of 92.8–96.4%. Strain BR-29T contained a cell wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The major fatty acids were anteiso-C15:0, C16:0 and iso-C16:0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, lysylphosphatidylglycerol and two unidentified phospholipids; a minor amount of phosphatidylglycerol was present. The DNA G+C content was 54.9 mol%. Strain BR-29T could be differentiated from phylogenetically related Cohnella species by differences in phenotypic characteristics. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BR-29T represents a novel species of the genus Cohnella, for which the name Cohnella boryungensis sp. nov., is proposed. The type strain is BR-29T (= KCTC 13735T = CCUG 59598T).

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Acknowledgments

This work was supported by the Program for Collection, Management and Utilization of Biological Resources (grant M10867010003) and BK21 program from the Ministry of Education, Science and Technology (MEST) of the Republic of Korea.

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Correspondence to Jung-Hoon Yoon.

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The GenBank accession number of the 16S rRNA gene sequence of Cohnella boryungensis BR-29T is HM061613.

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Yoon, JH., Jung, YT. Cohnella boryungensis sp. nov., isolated from soil. Antonie van Leeuwenhoek 101, 769–775 (2012). https://doi.org/10.1007/s10482-011-9691-x

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Keywords

  • Cohnella boryungensis
  • Novel species
  • Polyphasic taxonomy