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Aliidiomarina haloalkalitolerans sp. nov., a marine bacterium isolated from coastal surface seawater

Abstract

A novel Gram-negative, rod shaped, motile, non-sporing strictly aerobic bacterium, designated strain AK5T, was isolated from a sea water sample collected near Visakhapatnam coast, Bay of Bengal, India. Colonies on marine agar were circular, 3–4 mm in diameter, creamish and rose with entire margin. Growth occurred at 10–40°C, 0.5–12% (w/v) NaCl and pH of 7–11. Strain AK5T was oxidase and catalase positive. The fatty acids were dominated by iso-branched saturated and unsaturated fatty acids with a high abundance of iso-C15:0, iso-C17:0 and summed feature 9 (as defined by MIDI). Q8 was found to be the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and four unidentified phospholipids as polar lipids. The DNA G+C content of strain AK5T was 54.7 ± 0.2 mol%. Phylogenetic analysis revealed that strain AK5T was a member of the genus Aliidiomarina and closely related to Aliidiomarina taiwanensis with a phylogenetic distance of 5.3% (94.7% 16S rRNA gene sequence similarity) and clustered with the same species. Results from the polyphasic taxonomy study support the conclusion that strain AK5T represents a novel Aliidiomarina species, for which the name Aliidiomarina haloalkalitolerans sp. nov. is proposed. The type strain of A. haloalkalitolerans is AK5T (= MTCC 11064T = JCM 17359T).

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Acknowledgments

We thank Dr. J. Euzeby for his expert suggestion for the correct species epithet and Latin etymology. We also thank Council of Scientific and Industrial Research (CSIR) and Department of Biotechnology, Government of India for financial assistance. We would like to thank Mr. Deepak for his excellent help in sequencing DNA. The laboratory facility was extended by MMRF of NIO, RC, Kochi funded by the Ministry of Earth Sciences, New Delhi. TNRS is thankful to CSIR SIP projects (SIP1302 and SIP1308) for providing facilities and funding respectively.

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Correspondence to P. Anil Kumar.

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T. N. R Srinivas and P. Anil Kumar contributed equally to the present work.

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Srinivas, T.N.R., Nupur & Anil Kumar, P. Aliidiomarina haloalkalitolerans sp. nov., a marine bacterium isolated from coastal surface seawater. Antonie van Leeuwenhoek 101, 761–768 (2012). https://doi.org/10.1007/s10482-011-9690-y

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  • DOI: https://doi.org/10.1007/s10482-011-9690-y

Keywords

  • Aliidiomarina haloalkalitolerans
  • 16S rRNA gene based phylogeny
  • Chemotaxonomy