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Vibrio zhanjiangensis sp. nov., isolated from sea water of shrimp farming pond

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Abstract

A Gram-negative, facultatively anaerobic, motile by means of single polar flagellum, rod-shaped marine bacterium, designated strain E414, was isolated from sea water collected from a farming pond rearing marine shrimp Litopenaeus vannamei in Zhanjiang, Guangdong province, PRC. The strain was able to grow in the presence of 0.5–6% (w/v) NaCl (optimally in 3–6% (w/v) NaCl), between pH 6 and 9 (optimally at pH 7–8), between 15 and 37°C (optimally at 25–30°C). Phylogenetic analysis based on 16S rRNA gene sequences locate strain E414 in the vicinity of the coralliilyticus clade within the genus Vibrio. DNA–DNA relatedness data and multigene phylogenetic analysis based on the concatenated sequences of four genes (16S rRNA, rpoA, recA and pyrH) clearly differentiated strain E414 from its closest phylogenetic neighbours. Analysis of phenotypic features, including enzyme activities and utilization and fermentation of various carbon sources, further revealed discrimination between strain E414 and phylogenetically related Vibrio species. The major fatty acid components are C16:1ω6c and/or C16:1ω7c (27.4%), C18:1ω7c and/or C18:1ω6c (19.3%) and C16:0 (18.2%). The DNA G+C content of strain E414 was 38.7 mol%. Based on phenotypic, chemotaxonomic, phylogenetic and DNA–DNA relatedness values, it can be concluded that E414 should be placed in the genus Vibrio as representing a novel species, for which the name Vibrio zhanjiangensis sp. nov. is proposed, with the type strain E414 (=CCTCC AB 2011110T = NBRC 108723T = DSM 24901).

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Abbreviations

MLSA:

Multilocus sequence analysis

TCBS:

Thiosulfate-citrate-bile salts sucrose

O/129:

2,4-diamino-6,7-diisopropylpteridine

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Acknowledgments

This investigation was supported by Natural Science Foundation of China (30800857), Natural Science Foundation of Fujian Province (2010J05074), the Open Project Program of the Key Laboratory of Marine Bio-resources Sustainable Utilization, SCSIO, CAS (LMB101005), the Open Project Program of the Key Laboratory of Marine Biogenetic Resources, SOA (HY201102), the National Key Technology R&D Program (2009BAB44B02) and the Frontier Key Program for Youngsters in SCSIO (SQ200801). We are grateful to Ms Li Gu for her excellent technical assistance.

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Authors and Affiliations

  1. College of Chemical Engineering, Huaqiao University, Xiamen, 361021, People’s Republic of China

    Chunying Jin

  2. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, People’s Republic of China

    Huali Zuo, Jianming Chen, Mingliang Chen & Wei Wang

  3. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Guangzhou, 510301, People’s Republic of China

    Peng Luo

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  1. Chunying Jin
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  6. Wei Wang
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Correspondence to Chunying Jin or Wei Wang.

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Jin, C., Luo, P., Zuo, H. et al. Vibrio zhanjiangensis sp. nov., isolated from sea water of shrimp farming pond. Antonie van Leeuwenhoek 101, 743–751 (2012). https://doi.org/10.1007/s10482-011-9688-5

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