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Echinicola shivajiensis sp. nov., a novel bacterium of the family “Cyclobacteriaceae” isolated from brackish water pond

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Abstract

Strain AK12T, an orange pigmented Gram-negative, rod shaped, non-motile bacterium, was isolated from a mud sample collected from a brackish water pond at Rampur of West Bengal, India. The strain was positive for oxidase, catalase and phosphatase. The predominant fatty acids were iso-C15:0 (42.7%), iso-C17:0 3OH (13.2%), C16:1ω7c/C16:1ω6c (summed feature 3) (8.0%), iso-C17:1 I/anteiso-C17:1 B (summed feature 4) (6.1%) and iso-C17:1ω9c/C16:0 10-methyl (summed feature 9) (9.4%). Strain AK12T contained MK-7 as the major respiratory quinone and phosphatidylethanolamine, one unidentified aminophospholipid and six unidentified lipids as the polar lipids. The G + C content of DNA of the strain AK12T was 46.2 mol%. The 16S rRNA gene sequence analysis indicated that strain AK12T was member of the genus Echinicola and closely related to Echinicola vietnamensis, Echinicola pacifica and Echinicola jeungdonensis with pair-wise sequence similarity of 96.8, 96.3 and 96.0% respectively. Phylogenetic analyses indicated that the strain AK12T clustered with E. vietnamensis and together with E. pacifica and E. jeungdonensis with a phylogenetic distance of 5.1, 6.3 and 6.6% (94.9, 93.7 and 93.4% similarity) respectively. Based on data from the current polyphasic study, strain AK12T is proposed as a novel species of the genus Echinicola, for which the name Echinicola shivajiensis sp. nov. is proposed. The type strain of E. shivajiensis is AK12T (= MTCC 11083T = JCM 17847T).

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Acknowledgments

We thank Council of Scientific and Industrial Research (CSIR) and Department of Biotechnology, Government of India for financial assistance. We would like to thank Mr. Deepak for his excellent help in sequencing DNA. The laboratory facility was extended by MMRF of NIO, RC, Kochi funded by the Ministry of Earth Sciences, New Delhi. TNRS is thankful to CSIR SIP projects (SIP1302 and SIP1308) for providing facilities and funding respectively.

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Authors and Affiliations

  1. National Institute of Oceanography (CSIR), Regional Centre, P B No. 1913, Dr. Salim Ali Road, Kochi, 682018, Kerala, India

    T. N. R. Srinivas

  2. Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology (CSIR), Sector 39A, Chandigarh, 160 036, India

    P. Anil Kumar

  3. Institute of Microbial Technology (CSIR), Sector 39A, Chandigarh, 160 036, India

    B. Kailash Tryambak

Authors
  1. T. N. R. Srinivas
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  2. B. Kailash Tryambak
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  3. P. Anil Kumar
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Corresponding author

Correspondence to P. Anil Kumar.

Additional information

T. N. R. Srinivas and P. Anil Kumar contributed equally to this study.

Electronic supplementary material

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Supplementary Fig. S1. Phase contrast photograph of cells of strain AK12T. Bar, 5.0 μm (DOCX 153 kb)

10482_2011_9679_MOESM2_ESM.docx

Supplementary Fig. S2. Two-dimensional thin-layer chromatogram of polar lipids of the strains AK12T (a), LMG 23350T (b) and LMG 23754T (c). Detection of total lipids by molybdatophosphoric acid. Abbreviations: PE, phosphatidylethanolamine; AL, unidentified aminolipid; AP, unidentified aminophospholipid; L, unidentified lipid. (DOCX 336 kb)

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Srinivas, T.N.R., Kailash Tryambak, B. & Anil Kumar, P. Echinicola shivajiensis sp. nov., a novel bacterium of the family “Cyclobacteriaceae” isolated from brackish water pond. Antonie van Leeuwenhoek 101, 641–647 (2012). https://doi.org/10.1007/s10482-011-9679-6

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  • DOI: https://doi.org/10.1007/s10482-011-9679-6

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