Kibdelosporangium phytohabitans sp. nov., a novel endophytic actinomycete isolated from oil-seed plant Jatropha curcas L. containing 1-aminocyclopropane-1-carboxylic acid deaminase

An Erratum to this article was published on 27 October 2013

Abstract

A novel actinomycete, designated strain KLBMP 1111T, was isolated from the root of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China. Strain KLBMP 1111T formed a distinct branch in the 16S rRNA gene phylogenetic tree together with the type strains in the genus Kibdelosporangium, with the highest similarity to Kibdelosporangium aridum subsp. aridum DSM 43828T (98.8%), K. aridum subsp. largum DSM 44150T (98.1%) and Kibdelosporangium philippinense DSM 44226T (98.1%). The organism produced sporangium-like structures, the typical morphological characteristic of the genus Kibdelosporangium. The chemotaxonomic properties of this strain were also consistent with those of the genus Kibdelosporangium: the peptidoglycan contained meso-diaminopimelic acid; the predominant menaquinone was MK-9(H4); phospholipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol and an unknown phospholipid; iso-C16:0, C16:0, anteiso-C15:0 and iso-C15:0 as the predominant cellular fatty acids and the G+C content was 67.2 mol%. DNA–DNA hybridization values between strain KLBMP 1111T and the three Kibdelosporangium species were less than 50%. This strain had the ability to produce a siderophore, utilized 1-aminocyclopropane-1-carboxylic acid (ACC) as sole source of nitrogen and possessed ACC deaminase enzyme. Based on genotypic and phenotypic data, strain KLBMP 1111T represents a novel species in the genus Kibdelosporangium. We propose the name Kibdelosporangium phytohabitans sp. nov. for this species. The type strain is the strain KLBMP 1111T (=KCTC 19775T = CCTCC AA 2010001T).

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Acknowledgments

This research was partially supported by National Natural Science Foundation of China (Nos. 31000005, 31101502), the Program of Natural Science Foundation of the Jiangsu Higher Education Institutions of China (Nos. 11KJD210002, 10KJB180008), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the Project of Outstanding Scientific and Technological Innovation Team for Higher Education Institutions in Jiangsu Province (Pre-development of medical microbiology) and Grants from Natural Science Foundation by Xuzhou City (No. XZZD1004) and Xuzhou Normal University (09XLR12, 09XLR19).

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Correspondence to Sheng Qin or Ji-Hong Jiang.

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An erratum to this article is available at http://dx.doi.org/10.1007/s10482-013-0059-2.

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Xing, K., Bian, GK., Qin, S. et al. Kibdelosporangium phytohabitans sp. nov., a novel endophytic actinomycete isolated from oil-seed plant Jatropha curcas L. containing 1-aminocyclopropane-1-carboxylic acid deaminase. Antonie van Leeuwenhoek 101, 433–441 (2012). https://doi.org/10.1007/s10482-011-9652-4

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Keywords

  • Kibdelosporangium phytohabitans sp. nov.
  • Polyphasic taxonomy
  • 16S rRNA
  • 1-aminocyclopropane-1-carboxylic acid