Abstract
A novel Gram-positive, aerobic, motile, hemolytic, endospore-forming and rod-shaped bacterium TW25T was isolated from a dead ark clam during a mass mortality event on the South coast of Korea. The strain grew optimally at 30°C, at pH 8–9, and with 1% (w/v) NaCl. The 16S rRNA gene sequence analysis indicated that strain TW25T was associated with the genus Ornithinibacillus and that it was most closely related to the type strain of Ornithinibacillus californiensis (98.5% similarity). The dominant cellular fatty acids were iso-C15:0, anteiso-C15:0 and C16:0. The peptidoglycan amino acid type was A4β, containing l-ornithine and d-aspartic acid. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids, two unidentified aminolipids and two unidentified lipids. The major respiratory quinone was menaquinone-7 (MK-7). The G + C content of genomic DNA was 36.7 mol%. DNA–DNA hybridization experiments with related strains revealed lower than 11 ± 3% relatedness. Based on this polyphasic taxonomic study, strain TW25T represents a novel species in the genus Ornithinibacillus, for which the name Ornithinibacillus scapharcae sp. nov. is proposed. The type strain is TW25T (=KACC 15116T = JCM 17314T).
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Acknowledgments
We thank Dr. J. P. Euzéby (École Nationale Vétérinaire, France) for etymological advice. This work was supported by 21C Frontier Microbial Genomics and Application Center Program, and the grant from the National Fisheries Research and Development Institute (NFRDI), Republic of Korea. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain TW25T is HQ171440.
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Na-Ri Shin and Tae Woong Whon contributed equally to this work.
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10482_2011_9645_MOESM1_ESM.ppt
Supplementary Fig. S1. Phase contrast micrograph of strain TW25T after growth on TSA for 24 h, showing the endospore with swollen sporangia (A), and transmission electron micrograph of a cell of strain TW25T (B). Arrow indicates the peritrichous flagella of strain TW25T. (PPT 2995 kb)
10482_2011_9645_MOESM2_ESM.ppt
Supplementary Fig. S2. Two-dimensional thin-layer chromatograms of polar lipids from strain TW25T. Total lipids were revealed using Ornithinibacillus bavariensis WSBC 24001T. Abbreviations: DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PL1-4, unidentified phospholipids; AL1-2, unidentified aminolipids; L1-2, unidentified lipids. (PPT 239 kb)
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Shin, NR., Whon, T.W., Kim, MS. et al. Ornithinibacillus scapharcae sp. nov., isolated from a dead ark clam. Antonie van Leeuwenhoek 101, 147–154 (2012). https://doi.org/10.1007/s10482-011-9645-3
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DOI: https://doi.org/10.1007/s10482-011-9645-3