Bacteria associated with the pinewood nematode Bursaphelenchus xylophilus collected in Portugal

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In this study, we report on the bacterial community associated with the pinewood nematode Bursaphelenchus xylophilus from symptomatic pine wilted trees, as well as from long-term preserved B. xylophilus laboratory collection specimens, emphasizing the close bacteria–nematode associations that may contribute to pine wilt disease development.

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  1. Ashelford KE, Chuzhanova NA, Fry JC, Jones AJ, Weightman AJ (2005) At least 1 in 20 16S rRNA sequence records currently held in public repositories is estimated to contain substantial anomalies. Appl Environ Microbiol 71:7724–7736

    PubMed  Article  CAS  Google Scholar 

  2. Bai Y, Souleimanov A, Smith DL (2002) An inducible activator produced by a Serratia proteamaculans strain and its soybean growth-promoting activity under greenhouse conditions. J Exp Bot 53:1495–1502

    PubMed  Article  CAS  Google Scholar 

  3. Barash I, Manulis-Sasson S (2009) Recent evolution of bacterial pathogens: the gall-forming Pantoea agglomerans case. Annu Rev Phytopathol 47:133–152

    PubMed  Article  CAS  Google Scholar 

  4. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  5. Han ZM, Hong YD, Zhao BG (2003) A study on pathogenicity of bacteria carried by pine wood nematodes. J Phytopathol 151:683–689

    Article  Google Scholar 

  6. Hongoh Y, Yuzawa H, Ohkuma M, Kudo T (2003) Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural environment. FEMS Microbiol Lett 221:299–304

    PubMed  Article  CAS  Google Scholar 

  7. Kawazu K, Zhang H, Kanzaki H (1996a) Accumulation of benzoic acid in suspension cultured cells of Pinus thunbergii Parl. in response to phenylacetic acid administration. Biosci Biotechnol Biochem 60:1410–1412

    PubMed  Article  CAS  Google Scholar 

  8. Kawazu K, Zhang H, Yamashita H, Kanzaki H (1996b) Relationship between the pathogenicity of the pine wood nematode, Bursaphelenchus xylophilus, and phenylacetic acid production. Biosci Biotechnol Biochem 60:1413–1415

    PubMed  Article  CAS  Google Scholar 

  9. Kiyohara T, Tokushige Y (1971) Inoculation experiments of a nematode, Bursaphelenchus sp., onto pine trees. J Jpn For Sci 53:210–218

    Google Scholar 

  10. Kwon H, Choi G, Choi Y, Jang K, Sung N, Kang M, Moon Y, Lee S, Kim J (2010) Suppression of pine wilt disease by an antibacterial agent, oxolinic acid. Pest Manag Sci 66:634–639

    PubMed  CAS  Google Scholar 

  11. Laranjo M, Machado J, Young J, Oliveira S (2004) High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region. FEMS Microbiol Ecol 48:101–107

    PubMed  Article  CAS  Google Scholar 

  12. Li H (2007) Identification and pathogenicity of Bursaphelenchus species (Nematoda: Parasitaphelenchidae). Ph.D. thesis, University of Ghent

  13. Mohamed ZK, El-wahab GS (2009) Potency evaluation of Serratia marcescens and Pseudomonas fluorescens as biocontrol agents for root-knot nematodes in Egypt. J Appl Sci Res 4:93–102

    Google Scholar 

  14. Mota M, Braasch H, Bravo M, Penas A, Burgermeister W, Metge K, Sousa E (1999) First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1:727–734

    Article  Google Scholar 

  15. Oku H, Shiraishi T, Kurozumi S (1980) Pine wilt toxin, the metabolite of a bacterium associated with a nematode. Naturwissenschaften 67:198–199

    Article  CAS  Google Scholar 

  16. Proença DN, Francisco R, Santos CV, Lopes A, Fonseca L, Abrantes IM, Morais PV (2010) Diversity of bacteria associated with Bursaphelenchus xylophilus and other nematodes isolated from Pinus pinaster trees with pine wilt disease. PLoS ONE 5:e15191

    PubMed  Article  Google Scholar 

  17. Rodrigues J (2008) National eradication programme for pinewood nematode. In: Vieira P, Mota M (eds) Pine wilt disease: a worldwide threat to forest ecosystems. Springer, Dordrecht, pp 5–14

    Google Scholar 

  18. Staden R (1996) The Staden sequence analysis package. Mol Biotechnol 5:233–241

    PubMed  Article  CAS  Google Scholar 

  19. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    PubMed  Article  CAS  Google Scholar 

  20. Xie B, Cheng X, Shi J, Zhang Q, Dai S, Cheng F, Luo Y (2009) Mechanisms of invasive population establishment and spread of pinewood nematodes in China. Sci China C 52:587–594

    Article  Google Scholar 

  21. Zhao B, Lin F (2005) Mutualistic symbiosis between Bursaphelenchus xylophilus and bacteria of the genus Pseudomonas. For Pathol 35:339–345

    Article  Google Scholar 

  22. Zhao B, Ang H, An S, An Z (2003) Distribution and pathogenicity of bacteria species carried by Bursaphelenchus xylophilus in China. Nematology 5:899–906

    Article  Google Scholar 

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This work was supported by the Portuguese Project Programa de Acção Nacional para Controlo do Nemátodo da Madeira do Pinheiro: “A doença do nemátode da madeira do pinheiro Bursaphelenchus xylophilus” from Instituto de Financiamento da Agricultura e Pescas (IFAP) and Autoridade Florestal Nacional (AFN). The authors wish to thank Isabel Abrantes, Paula Morais and their research team from University of Coimbra (Coimbra, Portugal), for the support given in the sampling collection.

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Correspondence to Solange Oliveira.

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Vicente, C.S.L., Nascimento, F., Espada, M. et al. Bacteria associated with the pinewood nematode Bursaphelenchus xylophilus collected in Portugal. Antonie van Leeuwenhoek 100, 477–481 (2011).

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  • Pine wilt disease
  • Bursaphelenchus xylophilus
  • Nematode
  • Bacteria
  • Phylogeny