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Halomonas qijiaojingensis sp. nov. and Halomonas flava sp. nov., two moderately halophilic bacteria isolated from a salt lake

Antonie van Leeuwenhoek volume 100, pages 365–373 (2011)Cite this article

Abstract

Two moderately halophilic, Gram-negative, rod-shaped bacteria, designated YIM 93003T and YIM 94343T, were isolated from a salt lake in Xinjiang province, north-west China. The two strains YIM 93003T and YIM 94343T grew at 20–40°C, pH 6–9, 0.5–24% (w/v) NaCl and at 20–40°C, pH 6–9, 0.5–23% (w/v) NaCl, respectively. No growth occurred in absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains YIM 93003T and YIM 94343T were phylogenetically affiliated to the genus Halomonas and exhibited sequence similarity of 97.5% and 97.4% to the type strain Halomonas anticariensis DSM 16096T, respectively. The strains possessed chemotaxonomic markers that were consistent with their classification in the genus Halomonas (Q-9 as predominant respiratory quinine; C18:1ω7c, C16:0 and C16:1 ω7c/iso-C15:02-OH as the major fatty acids). The DNA–DNA hybridization values for strains YIM 93003T and YIM 94343T, YIM 93003T and DSM 16096T, YIM 94343T and DSM 16096T were 38.1 ± 3.0, 18.3 ± 4.7, and 20.8 ± 4.6%, respectively. The G+C contents of the strains YIM 93003T and YIM 94343T were 63.4 and 64.0 mol%, respectively. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including low DNA–DNA hybridization results, two novel species, Halomonas qijiaojingensis sp. nov., and Halomonas flava sp. nov., are proposed. The type strains are YIM 93003T (=CCTCC AB 208133T =KCTC 22228T) and YIM 94343T (=CCTCC AB 2010382T =KCTC 23356T), respectively.

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Acknowledgments

The authors are grateful to Prof. Hans-Peter Klenk for his kindly providing the reference type strain. This research was supported by National Basic Research Program of China (no. 2010CB833801) and Research Fund for the Doctoral Program of Higher Education of China (no. 200806730001).

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Authors and Affiliations

  1. Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, 843300, People’s Republic of China

    Chao Chen, Bing-Bing Liu, Hong-Zhuan Sun & Li-Li Zhang

  2. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Chao Chen, Rong Shi, Bing-Bing Liu, Yun-Jiao Zhang, Shu-Kun Tang & Wen-Jun Li

  3. Chinese Medicine Materials College, Jilin Agricultural University, Changchun, 130118, People’s Republic of China

    Chang-Tian Li

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  1. Chao Chen
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  2. Rong Shi
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Correspondence to Li-Li Zhang or Wen-Jun Li.

Additional information

Chao Chen, Rong Shi, and Bing-Bing Liu contributed equally to this work.

The GenBank accession numbers of 16S rRNA gene sequences of strains YIM 93003T and YIM 94343T are HQ832735 and HQ832736, respectively.

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Chen, C., Shi, R., Liu, BB. et al. Halomonas qijiaojingensis sp. nov. and Halomonas flava sp. nov., two moderately halophilic bacteria isolated from a salt lake. Antonie van Leeuwenhoek 100, 365–373 (2011). https://doi.org/10.1007/s10482-011-9591-0

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Keywords

  • Halomonas qijiaojingensis sp. nov
  • Halomonas flava sp. nov
  • Polyphasic taxonomy
  • 16S rRNA gene