Skip to main content

Glycomyces halotolerans sp. nov., a novel actinomycete isolated from a hypersaline habitat in Xinjiang, China

Abstract

A novel actinomycete strain, designated TRM 40137T, was isolated from a hypersaline habitat in Xinjiang Province, north-west China, and subjected to a polyphasic taxonomic study. The strain was aerobic, Gram-positive and the optimum NaCl concentration for growth was 4–5% (w/v). Phylogenetic analysis showed that strain TRM 40137T has a 16S rRNA gene sequence similarity of 95.02% with the described species Glycomyces sambucus E71T and can be distinguished from all previously described representatives of the genus Glycomyces. The whole-cell sugar pattern consisted of xylose and galactose. The predominant menaquinone was MK-10(H2) and the major fatty acids were anteiso-C15:0 and anteiso-C17:0. The phospholipid pattern consists of phosphatidylglycerol, diphosphatidylglycerol, three unknown aminophospholipids and two unknown phospholipids. The G+C content of the genomic DNA was 68.8 mol%. A novel species Glycomyces halotolerans sp. nov. is proposed, with strain TRM 40137T (=CCTCC AA 2010013T = KCTC 19988T) as the type strain of G. halotolerans.

This is a preview of subscription content, access via your institution.

Fig. 1

References

  1. Chun J, Lee J-H, Jung Y, Kim M, Kim S, Kim B-K, Lim Y-W (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261

    PubMed  Article  CAS  Google Scholar 

  2. Collins M-D, Pirouz T, Goodfellow M, Minnikin D-E (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    PubMed  CAS  Google Scholar 

  3. Cui X-L, Mao P-H, Tseng M, Li W-J, Zhang L-P, Xu L-H, Jiang C-L (2001) Streptomonospora salina gen. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51:357–363

    PubMed  CAS  Google Scholar 

  4. Evtushenko LI, Taptykova SD, Akimov VN, Semyonova SA, Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  Google Scholar 

  5. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789

    Article  Google Scholar 

  6. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  7. Gordon RE, Barnett DA, Handerhan JE, Pang CH-N (1974) Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63

    Article  Google Scholar 

  8. Groth I, Schumann P, Rainey FA, Martin K, Schuetze B, Augsten K (1997) Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133

    PubMed  Article  CAS  Google Scholar 

  9. Gu Q, Zheng W, Huang Y (2007) Glycomyces sambucus sp. nov., an endophytic actinomycete isolated from the stem of Sambucus adnata wall. Int J Syst Evol Microbiol 57:1995–1998

    PubMed  Article  CAS  Google Scholar 

  10. Guan T-W, Tang S-K, Wu J-Y, Zhi X-Y, Xu L-H, Li W-J, Zhang L-L (2009) Haloglycomyces albus gen. nov., sp. nov., a novel Halophilic filamentous actinomycete of the family Glycomycetaceae. Int J Syst Evol Microbiol 59:1297–1301

    PubMed  Article  CAS  Google Scholar 

  11. Guan T-W, Xiao J, Zhao K, Luo X-X, Zhang X-P, Zhang L-L (2010) Halomonas xinjiangensis sp. nov., a halotolerant bacterium isolated from a salt lake in Xinjiang, China. Int J Syst Evol Microbiol 60:349–352

    PubMed  Article  CAS  Google Scholar 

  12. Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322

    Article  CAS  Google Scholar 

  13. Kelly KL (1964) Inter-society color council-national bureau of standards color name charts illustrated with centroid colors. US Government Printing Office, Washington

    Google Scholar 

  14. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16:111–120

    PubMed  Article  CAS  Google Scholar 

  15. Labeda DP, Kroppenstedt RM (2004) Emended description of the genus Glycomyces and description of Glycomyces algeriensis sp. nov., Glycomyces arizonensis sp. nov. and Glycomyces lechevalierae sp. nov. Int J Syst Evol Microbiol 54:2343–2346

    PubMed  Article  Google Scholar 

  16. Labeda DP, Kroppenstedt RM (2005) Stackebrandtia nassauensis gen. nov., sp. nov. and emended description of the family Glycomycetaceae. Int J Syst Evol Microbiol 55:1687–1691

    PubMed  Article  CAS  Google Scholar 

  17. Labeda DP, Testa RT, Lechevalier MP, Lechevalier HA (1985) Glycomyces, a new genus of the Actinomycetales. Int J Syst Bacteriol 35:417–421

    Article  CAS  Google Scholar 

  18. Lee SD (2006) Brevibacterium samyangense sp. nov., an actinomycete isolated from a beach sediment. Int J Syst Evol Microbiol 56:1889–1892

    PubMed  Article  CAS  Google Scholar 

  19. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218

    Article  CAS  Google Scholar 

  20. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  21. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of isoprenoid quinines and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  22. Qin S, Wang H-B, Chen H-H, Zhang Y-Q, Jiang C-L, Xu L-H, Li W-J (2008) Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. Int J Syst Evol Microbiol 58:2525–2528

    PubMed  Article  CAS  Google Scholar 

  23. Qin S, Chen H-H, Klenk HP, Zhao G-Z, Li J, Xu L-H, Li W-J (2009) Glycomyces scopariae sp. nov. and Glycomyces mayteni sp. nov., isolated from medicinal plants in China. Int J Syst Evol Microbiol 59:1023–1027

    PubMed  Article  CAS  Google Scholar 

  24. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  25. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc, Newwark

    Google Scholar 

  26. Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  27. Stackebrandt E, Rainey FA, Ward-Rainey NL (1997) Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491

    Article  Google Scholar 

  28. Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231

    PubMed  CAS  Google Scholar 

  29. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol and Evol 24:1596–1599

    Article  CAS  Google Scholar 

  30. Wang Y-X, Zhi X-Y, Zhang Y-Q, Cui X-L, Xu L-H, Li W-J (2009) Stackebrandtia albiflava sp. nov. and emended description of the genus Stackebrandtia. Int J Syst Evol Microbiol 59:574–577

    PubMed  Article  CAS  Google Scholar 

  31. Williams ST (1967) Sensitivity of streptomycetes to antibiotics as a taxonomic character. J Gen Microbiol 46:151–160

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by National Natural Science Foundation of China (Project no. 31060003, 31060001), Key Project for Department of Science and Technology of Ministry of Education of China (Project no. 209145), Opening Project by State Key Laboratory of Microbial Resources. Institute of Microbiology, Chinese Academy of Sciences (Project no. SKLMR-20090603), The Opening Project of Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences (Project no. LBB-2010-004) and 973 Program (Project no. 2010CB134505).

Author information

Affiliations

Authors

Corresponding authors

Correspondence to Tong-Wei Guan or Xiao-Ping Zhang.

Additional information

The 16S rRNA gene sequence of strain TRM 40137T has been deposited in GenBank under the accession number HQ651156.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 2857 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Guan, TW., Xia, ZF., Xiao, J. et al. Glycomyces halotolerans sp. nov., a novel actinomycete isolated from a hypersaline habitat in Xinjiang, China. Antonie van Leeuwenhoek 100, 137–143 (2011). https://doi.org/10.1007/s10482-011-9574-1

Download citation

Keywords

  • Glycomyces halotolerans sp. nov.
  • Polyphasic taxonomy
  • 16S rRNA