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Gilvimarinus agarilyticus sp. nov., a new agar-degrading bacterium isolated from the seashore of Jeju Island

Abstract

An agarolytic bacterium, designated as strain M5cT, was isolated from sea sand in Jeju Island, Korea. This isolate was Gram-negative, positive for catalase and oxidase, rod and motile by means of monotrichous flagella. Strain M5cT has translucent or dark ivory colonies, forms a dent on an agar plate under colonies, and grows in the presence of 1–12% (w/v) NaCl and at 10–37°C. This isolate hydrolyzes agar, alginic acid, carboxymethyl (CM)-cellulose and starch. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M5cT can be considered as a species within the genus Gilvimarinus, being most closely related to Gilvimarinus chinensis QM42T, with a 16S rRNA gene sequence similarity of 95.6%. The major cellular fatty acids were C16:1ω7c and/or iso-C15:0 2OH (33.5%), C16:0 (26.5%) and C18:1ω7c (14.1%). The DNA G+C content was 53.8 mol%. Based on these polyphasic data, strain M5cT should be classified as a novel species, for which the name Gilvimarinus agarilyticus sp. nov. is proposed. The type strain for the novel species is M5cT (= KCTC 23325T = NCAIM B 02425T).

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Acknowledgments

We thank Prof. Hans Georg Trüper for his advice on the Latin naming of the organism. This work was supported by a grant (NMC0301039) from the Ministry of Science and Technology (MOST) of the Republic of Korea and by a grant from the KRIBB Research Initiative Program.

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Correspondence to Kee-Sun Shin.

Additional information

The subject category: new taxa (other Gram-negative bacteria).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain M5cT is GQ872424.

Maximum-likelihood and Maximum-parsimony trees, based on 16S rRNA gene sequences of Gilvimarinus agarilyticus M5cT and related species, a transmission electron micrograph of G. agarilyticus M5cT and a table for fatty acid compositions of strain M5cT and G. chinensis QM42T are available as supplementary materials in Antonie van Leeuwenhoek Online.

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Kim, BC., Kim, M.N., Lee, K.H. et al. Gilvimarinus agarilyticus sp. nov., a new agar-degrading bacterium isolated from the seashore of Jeju Island. Antonie van Leeuwenhoek 100, 67–73 (2011). https://doi.org/10.1007/s10482-011-9565-2

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  • DOI: https://doi.org/10.1007/s10482-011-9565-2

Keywords

  • Gilvimarinus agarilyticus
  • Agar
  • Phylogeny
  • Taxonomy
  • 16S rRNA gene