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Gilvimarinus agarilyticus sp. nov., a new agar-degrading bacterium isolated from the seashore of Jeju Island

Antonie van Leeuwenhoek volume 100, pages 67–73 (2011)Cite this article

Abstract

An agarolytic bacterium, designated as strain M5cT, was isolated from sea sand in Jeju Island, Korea. This isolate was Gram-negative, positive for catalase and oxidase, rod and motile by means of monotrichous flagella. Strain M5cT has translucent or dark ivory colonies, forms a dent on an agar plate under colonies, and grows in the presence of 1–12% (w/v) NaCl and at 10–37°C. This isolate hydrolyzes agar, alginic acid, carboxymethyl (CM)-cellulose and starch. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M5cT can be considered as a species within the genus Gilvimarinus, being most closely related to Gilvimarinus chinensis QM42T, with a 16S rRNA gene sequence similarity of 95.6%. The major cellular fatty acids were C16:1ω7c and/or iso-C15:0 2OH (33.5%), C16:0 (26.5%) and C18:1ω7c (14.1%). The DNA G+C content was 53.8 mol%. Based on these polyphasic data, strain M5cT should be classified as a novel species, for which the name Gilvimarinus agarilyticus sp. nov. is proposed. The type strain for the novel species is M5cT (= KCTC 23325T = NCAIM B 02425T).

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Acknowledgments

We thank Prof. Hans Georg Trüper for his advice on the Latin naming of the organism. This work was supported by a grant (NMC0301039) from the Ministry of Science and Technology (MOST) of the Republic of Korea and by a grant from the KRIBB Research Initiative Program.

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Authors and Affiliations

  1. Microbiological Resources Center, KRIBB, Daejeon, 305-806, Republic of Korea

    Byung-Chun Kim, Mi Na Kim, Kang Hyun Lee & Kee-Sun Shin

  2. Plant Systems Engineering Research Center, KRIBB, Daejeon, 305-806, Republic of Korea

    Hyun Soon Kim & Sung Ran Min

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  1. Byung-Chun Kim
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  2. Mi Na Kim
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  3. Kang Hyun Lee
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  4. Hyun Soon Kim
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Correspondence to Kee-Sun Shin.

Additional information

The subject category: new taxa (other Gram-negative bacteria).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain M5cT is GQ872424.

Maximum-likelihood and Maximum-parsimony trees, based on 16S rRNA gene sequences of Gilvimarinus agarilyticus M5cT and related species, a transmission electron micrograph of G. agarilyticus M5cT and a table for fatty acid compositions of strain M5cT and G. chinensis QM42T are available as supplementary materials in Antonie van Leeuwenhoek Online.

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Kim, BC., Kim, M.N., Lee, K.H. et al. Gilvimarinus agarilyticus sp. nov., a new agar-degrading bacterium isolated from the seashore of Jeju Island. Antonie van Leeuwenhoek 100, 67–73 (2011). https://doi.org/10.1007/s10482-011-9565-2

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  • DOI: https://doi.org/10.1007/s10482-011-9565-2

Keywords

  • Gilvimarinus agarilyticus
  • Agar
  • Phylogeny
  • Taxonomy
  • 16S rRNA gene