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Rhizobium sphaerophysae sp. nov., a novel species isolated from root nodules of Sphaerophysa salsula in China

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Abstract

Four gram-negative, aerobic, motile, non-spore, forming rods with a wide pH and temperature range for growth (pH 7.0–11.0, optimum pH 8.0; 20–45°C, optimum 28°C) strains were isolated from root nodules of Sphaerophysa salsula and characterized by means of a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the four strains formed a new lineage related to the genus Rhizobium and the sequence similarities between the isolate and the most related type strain Rhizobium giardinii was 96.5%. These strains also formed a distinctive group from the reference strains for defined Rhizobium species based on housekeeping gene sequences (atpD and recA), BOX-PCR fingerprinting, phenotypic features and symbiotic properties. The representative strain CCNWGS0238T has DNA-DNA relatedness of less than 33.4% with the most closely related species R. giardinii. It is therefore proposed as a new species, Rhizobium sphaerophysae sp. nov., with isolate CCNWGS0238T (=ACCC17498T = HAMBI3074T) as the type strain.

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Acknowledgments

This work was supported by projects from the 973 Project of China (2010CB126502), National Science Foundation of China (30970003, 30900215 and 30630054), International Science & Technology Cooperation Program of China (2010DFA91930). We are grateful to Dr. Kristina Lindström and Prof. Ruibo Jiang for deposition of the strain in the culture collections.

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Authors and Affiliations

  1. College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi, 712100, China

    Lin Xu, Peng Zhao, Wei Min Chen, Wei Qin, Ming Tang & Ge Hong Wei

  2. Department of Life Science and Engineering, Hexi University, Zhangye Gansu, 734000, China

    Lin Xu & Jian Feng Shi

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  1. Lin Xu
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  2. Jian Feng Shi
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  3. Peng Zhao
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  4. Wei Min Chen
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  5. Wei Qin
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  6. Ming Tang
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  7. Ge Hong Wei
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Corresponding author

Correspondence to Ge Hong Wei.

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Xu, L., Shi, J.F., Zhao, P. et al. Rhizobium sphaerophysae sp. nov., a novel species isolated from root nodules of Sphaerophysa salsula in China. Antonie van Leeuwenhoek 99, 845–854 (2011). https://doi.org/10.1007/s10482-011-9559-0

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