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Halomonas aidingensis sp. nov., a moderately halophilic bacterium isolated from Aiding salt lake in Xinjiang, China

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Abstract

Two Gram-negative moderately halophilic bacterial strains, designated Ad-1T and C-12, were isolated from Aiding salt lake of Xinjiang in China. The novel isolates were subjected to a polyphasic taxonomic study. Cells of these strains were cocci or short rods and motile with polar flagella. Colonies produced brown-red pigment. The isolates grew in the range of 0.5–25% (w/v) NaCl, pH 5.5–10.5 and 4–45°C. Analysis of their 16S rRNA gene sequences indicated that strains Ad-1T and C-12 belonged to the genus Halomonas showing 92.7–98.4% similarity with the type species. The isoprenoid quinones of the isolates were Q-9 and Q-8. The major cellular fatty acids were C18:1ω7c, C16:1ω7c/6c, C16:0, C12:0–3OH and C10:0. The DNA G + C contents of strains Ad-1T and C-12 were 64.6 and 63.9 mol%, respectively. The DNA relatedness between the two isolates was 89.2%. The similarities of these newly isolated strains with closely related type strains were lower than 35% at the genetic level. Based on phenotypic, chemotaxonomic and genetic characteristics, the representative strain Ad-1T is considered to be a novel species of the genus Halomonas, for which the name Halomonas aidingensis sp. nov. is proposed, with Ad-1T (= CGMCC 1.10191T = NBRC 106173T) as the type strain.

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Acknowledgments

This work was supported financially by the Program for New Century Excellent Talents in Xiamen University to Prof. Chun Fan. We are grateful to Yuguang Zhou (China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences), for helping in microbiological culture collection.

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Authors and Affiliations

  1. National Engineering Laboratory of Biohydrometallurgy, General Research Institute for Nonferrous Metals, Beijing, 100088, People’s Republic of China

    Wen-Yan Liu

  2. Department of Preventive Medicine, Medical College of Xiamen University, Xiamen, 361005, People’s Republic of China

    Juan Wang

  3. Nanshan District Center for Disease Control and Prevention, Shenzhen, Guangdong, 518054, People’s Republic of China

    Meng Yuan

Authors
  1. Wen-Yan Liu
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  2. Juan Wang
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  3. Meng Yuan
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Corresponding authors

Correspondence to Wen-Yan Liu or Juan Wang.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strains Ad-1T and C-12 are GQ281062 and GU377268.

These two authors are contributed equally to this work: Juan Wang and Wen-Yan Liu.

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Supplementary Figure S1

Supplementary Figure S1. Transmission electron micrograph of strain Ad-1T. Bar, 1 μm Supplementary Figure S2 (a & b) Supplementary Figure S2. Phylogenetic tree based on 16S rRNA gene sequences. Numbers at branching points refer to bootstrap values (1000 resamplings; only values above 50% are shown). (a) minimum-evolution tree. (b) maximum-parsimony tree. (PDF 1047 kb)

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Liu, WY., Wang, J. & Yuan, M. Halomonas aidingensis sp. nov., a moderately halophilic bacterium isolated from Aiding salt lake in Xinjiang, China. Antonie van Leeuwenhoek 99, 663–670 (2011). https://doi.org/10.1007/s10482-010-9542-1

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  • DOI: https://doi.org/10.1007/s10482-010-9542-1

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