Methylobacterium soli sp. nov. a methanol-utilizing bacterium isolated from the forest soil

Abstract

A Gram-negative, pink-pigmented, non-spore-forming rod shaped, methanol-utilizing bacterium, strain YIM 48816T, was isolated from forest soil collected from Sichuan province, China. Strain YIM 48816T can grow at 4–37°C, pH 5.0–7.0 and 0% NaCl (w/v). Based on 16S rRNA gene sequence similarity studies, it belonged to the genus Methylobacterium, and formed a phyletic line. The 16S rRNA gene sequence similarities were 96.2% to Methylobacterium mesophilicum DSM 1708T and 96.0% to Methylobacterium brachiatum DSM 19569T, and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 96.0%. The major menaquinones detected were Q-10 (97.14%) and Q-9 (2.86%). The major fatty acids were C18:1 ω7c (80.84%). The DNA G + C content was 66.2 mol%. It is apparent from the genotypic and phenotypic data that strain YIM 48816T belongs to a novel species of the genus Methylobacterium, for which the name Methylobacterium soli sp. nov. is proposed. The type strain is YIM 48816T (CCTCC AA 208027T = KCTC 22810T).

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2

References

  1. Austin B, Goodfellow M (1979) Pseudomonas mesophilica, a new species of pink bacteria isolated from leaf surfaces. Int J Syst Bacteriol 29:373–378

    Article  Google Scholar 

  2. Cerny G (1978) Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. Appl Microbiol Biotechnol 5:113–122

    CAS  Article  Google Scholar 

  3. Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261

    CAS  Article  PubMed  Google Scholar 

  4. Cowan ST, Steel KJ (1965) Manual for the identification of medical bacteria. Cambridge University Press, London

    Google Scholar 

  5. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    CAS  Article  PubMed  Google Scholar 

  6. Felsenstein J (1985) Confidence limits on phylogenies: approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  7. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  8. Gordon RE, Barnett DA, Handerhan JE, Pang C (1974) Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Evol Microbiol 24:54–63

    Google Scholar 

  9. Green PN, Bousfield IJ (1983) Emendation of Methylobacterium Patt, Cole, Hanson 1976 Methylobacterium rhodinum (Heumann 1962) comb. nov. corrig., Methylobacterium radiotolerans (Ito, Iizuka 1971) comb. nov. corrig., Methylobacterium mesophilicum (Austin, Goodfellow 1979 comb. nov. Int J Syst Bacteriol 33:875–877

    Article  Google Scholar 

  10. Kato Y, Asahara M, Goto K, Kasai H, Yokota A (2008) Methylobacterium persicinum sp. nov., Methylobacterium komagatae sp. nov., Methylobacterium brachiatum sp. nov., Methylobacterium tardum sp. nov. and Methylobacterium gregans sp. nov., isolated from freshwater. Int J Syst Evol Microbiol 58:1134–1141

    CAS  Article  PubMed  Google Scholar 

  11. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16:111–120

    CAS  Article  PubMed  Google Scholar 

  12. Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207

    CAS  Article  Google Scholar 

  13. Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinformatics 5:150–163

    CAS  Article  PubMed  Google Scholar 

  14. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428

    Article  PubMed  Google Scholar 

  15. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    CAS  Article  Google Scholar 

  16. Patt TE, Cole GC, Hanson RS (1976) Methylobacterium, a new genus of facultatively methylotrophic bacteria. Int J Syst Evol Microbiol 26:226–229

    CAS  Google Scholar 

  17. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  18. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:1–6

    Google Scholar 

  19. Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  20. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    CAS  Article  PubMed  Google Scholar 

  21. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu LH, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153

    CAS  Article  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 30900002 and No. 30560001), International Cooperative Key Project of Ministry of Science and Technology (2006DFA33550). Authors are grateful to Yong-xia Wang and Yun Wang for their help in the study.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Yi Jiang.

Additional information

The GenBank accession number for the 16S rRNA gene sequence of strain YIM 48816T is EU860984.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Cao, YR., Wang, Q., Jin, RX. et al. Methylobacterium soli sp. nov. a methanol-utilizing bacterium isolated from the forest soil. Antonie van Leeuwenhoek 99, 629–634 (2011). https://doi.org/10.1007/s10482-010-9535-0

Download citation

Keywords

  • Methylobacteriumsoli sp. nov
  • Soil
  • 16S rRNA gene