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Inhibition of Penicillium digitatum and Penicillium italicum in vitro and in planta with Panomycocin, a novel exo-β-1,3-glucanase isolated from Pichia anomala NCYC 434

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Abstract

Panomycocin, a novel exo-beta 1,3 glucanase, was tested as an antifungal agent against green and blue mold diseases, the most important causes of post harvest decay in citrus fruits. All tested isolates of Penicillium digitatum and Penicillium italicum were susceptible to panomycocin in vitro. Effective panomycocin concentrations for 50% growth inhibition (MIC-2) for P. digitatum and P. italicum were 2 and 1 μg ml−1, respectively. Complete (MIC-0) growth inhibition of all isolates observed at a panomycocin concentration of 16 μg ml−1. Treatment of spores with panomycocin at values lower than the MIC-0 led to slower germ tube elongation and mycelium growth. In tests on fruit, panomycocin at concentrations equal to in vitro MIC-0 value protected lemon fruit from decay.

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Acknowledgments

This work was supported by a grant from the Scientific and Technological Research Council of Turkey (TUBITAK) (Project no: 104T495).

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Authors and Affiliations

  1. Department of Biology, TED Ankara College Foundation High School, Incek, Gölbaşı, 06830, Ankara, Turkey

    Demet Altınbay Izgu

  2. Department of Biological Sciences, Middle East Technical University, 06531, Ankara, Turkey

    Remziye Aysun Kepekci & Fatih Izgu

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  1. Demet Altınbay Izgu
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  2. Remziye Aysun Kepekci
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  3. Fatih Izgu
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Correspondence to Fatih Izgu.

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Izgu, D.A., Kepekci, R.A. & Izgu, F. Inhibition of Penicillium digitatum and Penicillium italicum in vitro and in planta with Panomycocin, a novel exo-β-1,3-glucanase isolated from Pichia anomala NCYC 434. Antonie van Leeuwenhoek 99, 85–91 (2011). https://doi.org/10.1007/s10482-010-9527-0

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