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Paracoccus niistensis sp. nov., isolated from forest soil, India

Abstract

A Gram-negative, non-motile, catalase-positive and oxidase-positive, aerobic bacterium designated as NII-0918T was isolated from soil sample in Western ghat forest, India. 16S rRNA gene sequence analysis showed that strain NII-0918T belongs to the subclass α-Proteobacteria, being related to the genus Paracoccus, and sharing highest sequence similarity with Paracoccus chinensis NBRC 104937T (99.4%), Paracoccus marinus NBRC 100640T (97.3%), Paracoccus koreensis Ch05T (97.1%) and Paracoccus kondratievae GBT (97.0%). Other members of Paracoccus showed below 97.0% similarity. The DNA–DNA hybridization values between these four strains and NII-0918T were 44.7, 28, 32 and 41%, respectively. The major fatty acids of strain NII-0918T were summed feature 7 (C18:1 ω7c/ω 9t/ω 12t) (83.0%) and C18:0 (12.5%). Ubiquinone Q-10 was detected as the major respiratory quinone. The G+C content of genomic DNA of NII-0918T was 66.6 mol%. On the basis of physiological, morphological, chemotaxonomical and DNA–DNA hybridization data, it is proposed that strain NII-0918T should be placed as a novel species, for which we propose Paracoccus niistensis sp. nov. The type strain is NII-0918T (CCTCC AA 209055T = NCIM 5340T = KCTC 22789T).

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Acknowledgment

The authors would like to thank CSIR Task force network programme on Exploration of India’s Rich Microbial Diversity (NWP 0006) for providing the financial support.

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Correspondence to Syed G. Dastager.

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Dastager, S.G., Deepa, C.K., Li, WJ. et al. Paracoccus niistensis sp. nov., isolated from forest soil, India. Antonie van Leeuwenhoek 99, 501–506 (2011). https://doi.org/10.1007/s10482-010-9515-4

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  • DOI: https://doi.org/10.1007/s10482-010-9515-4

Keywords

  • Paracoccus niistensis sp. nov
  • Polyphasic taxonomy
  • 16S rRNA